Sampson Nicole S, Vrielink Alice
Department of Chemistry, State University of New York, Stony Brook, New York 11794-3400, USA.
Acc Chem Res. 2003 Sep;36(9):713-22. doi: 10.1021/ar9800587.
Cholesterol oxidases are important as clinical reagents, potential larvicides, and tools in cell biology, and they are implicated in bacterial pathogenesis. Here we review chemical aspects of their function. We describe our current structural and mechanistic understanding of the type I and II cholesterol oxidases, our identification of an NH-pi hydrogen bond motif for stabilization of reduced flavins, our structural hypothesis of how O(2) gains access to the flavin, and our present understanding of type I cholesterol oxidase-lipid bilayer interactions.
胆固醇氧化酶作为临床试剂、潜在的杀幼虫剂以及细胞生物学工具具有重要意义，并且它们与细菌发病机制有关。在此我们综述其功能的化学方面。我们描述了目前对I型和II型胆固醇氧化酶的结构和作用机制的理解，我们对用于稳定还原黄素的NH-π氢键基序的鉴定，关于氧气如何接近黄素的结构假说，以及我们目前对I型胆固醇氧化酶-脂质双层相互作用的理解。
