Wu Baoyan, Zhao Na, Hou Shihua, Zhang Cong
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China.
Nanomaterials (Basel). 2016 Nov 21;6(11):220. doi: 10.3390/nano6110220.
Here we report a facile eco-friendly one-step electrochemical approach for the fabrication of a polypyrrole (PPy), reduced graphene oxide (RGO), and gold nanoparticles (nanoAu) biocomposite on a glassy carbon electrode (GCE). The electrochemical behaviors of PPy-RGO-nanoAu and its application to electrochemical detection of hydrogen peroxide were investigated by cyclic voltammetry. Graphene oxide and pyrrole monomer were first mixed and casted on the surface of a cleaned GCE. After an electrochemical processing consisting of the electrooxidation of pyrrole monomer and simultaneous electroreduction of graphene oxide and auric ions (Au) in aqueous solution, a PPy-RGO-nanoAu biocomposite was synthesized on GCE. Each component of PPy-RGO-nanoAu is electroactive without non-electroactive substance. The obtained PPy-RGO-nanoAu/GCE exhibited high electrocatalytic activity toward hydrogen peroxide, which allows the detection of hydrogen peroxide at a negative potential of about -0.62 V vs. SCE. The amperometric responses of the biosensor displayed a sensitivity of 40 µA/mM, a linear range of 32 µM-2 mM, and a detection limit of 2.7 µM (signal-to-noise ratio = 3) with good stability and acceptable reproducibility and selectivity. The results clearly demonstrate the potential of the as-prepared PPy-RGO-nanoAu biocomposite for use as a highly electroactive matrix for an amperometric biosensor.
在此，我们报道了一种简便的、环保的一步电化学方法，用于在玻碳电极（GCE）上制备聚吡咯（PPy）、还原氧化石墨烯（RGO）和金纳米颗粒（nanoAu）生物复合材料。采用循环伏安法研究了PPy-RGO-nanoAu的电化学行为及其在过氧化氢电化学检测中的应用。首先将氧化石墨烯和吡咯单体混合并浇铸在清洁后的GCE表面。经过由吡咯单体的电氧化以及氧化石墨烯和金离子（Au）在水溶液中的同步电还原组成的电化学处理后，在GCE上合成了PPy-RGO-nanoAu生物复合材料。PPy-RGO-nanoAu的每个组分都具有电活性，不存在非电活性物质。所制备的PPy-RGO-nanoAu/GCE对过氧化氢表现出高电催化活性，这使得能够在相对于饱和甘汞电极（SCE）约 -0.62 V的负电位下检测过氧化氢。该生物传感器的安培响应显示出40 μA/mM的灵敏度、32 μM - 2 mM的线性范围以及2.7 μM的检测限（信噪比 = 3），具有良好的稳定性以及可接受的重现性和选择性。结果清楚地证明了所制备的PPy-RGO-nanoAu生物复合材料作为安培生物传感器的高电活性基质的潜力。
