Schaumann R F, Shah P M
Infektiologie, Zentrum der Inneren Medizin, Klinikum der J.W. Goethe-Universität, Frankfurt, Germany.
Methods Find Exp Clin Pharmacol. 1992 Dec;14(10):753-8.
The influence of amphotericin B and rifampicin alone and in combination on the killing of Candida albicans, Candida tropicalis and Cryptococcus neoformans by human polymorphonuclear leukocytes was investigated in vitro. Granulocytes were harvested from the blood of healthy volunteers and resuspended with the above mentioned fungi. After 6 hours, 81.5% of C. albicans, 97% of C. tropicalis but only 34% of C. neoformans were killed. The activity of amphotericin B against C. albicans and C. tropicalis was better in granulocyte-free medium. A synergism between amphotericin B and rifampicin towards Candida could be detected. It was also demonstrated in the presence of polymorphonuclear leukocytes with a factor > 1 log in reduction of colony count. However, whether granulocytes were present or not, rifampicin (4 mcg/mL) alone did not exert any influence on growth of Candida.
在体外研究了两性霉素B和利福平单独及联合使用对人多形核白细胞杀死白色念珠菌、热带念珠菌和新型隐球菌的影响。从健康志愿者血液中采集粒细胞，并与上述真菌重悬。6小时后，81.5%的白色念珠菌、97%的热带念珠菌被杀死，但仅34%的新型隐球菌被杀死。两性霉素B在无粒细胞培养基中对白色念珠菌和热带念珠菌的活性更好。可检测到两性霉素B和利福平对念珠菌有协同作用。在多形核白细胞存在的情况下也得到证实，菌落计数减少了1个对数以上。然而，无论是否存在粒细胞，单独使用利福平（4微克/毫升）对念珠菌的生长均无任何影响。
