Del Pozo Jose L, Francés María L, Hernáez Silvia, Serrera Alicia, Alonso Marta, Rubio Manuel F
Infectious Diseases Division, Clínica Universidad de Navarra, Pamplona, Spain.
Int J Artif Organs. 2011 Sep;34(9):766-70. doi: 10.5301/ijao.5000023.
Effectiveness of amphotericin B alone or in combination with rifampicin or clarithromycin on the killing of Candida species biofilms was investigated in vitro. Amphotericin B was assayed at 0.005 to 10 mg/ml. Rifampin and clarithromycin were assayed at 10 mg/ml. We studied 7 Candida albicans, 3 Candida parapsilosis, 3 Candida glabrata, 3 Candida krusei and 2 Candida tropicalis strains. Biofilms were developed in 96-well, flat-bottomed microtiter plates for 48 hours. A synergistic effect between amphotericin B and clarithromycin was demonstrated against 66.6% of C. parapsilosis, 66.6% of C. glabrata, and 42.8% of C. albicans biofilms. A synergistic effect between amphotericin B and rifampin was demonstrated against 66.6% of C. parapsilosis, 42.8% of C. albicans, and 33.3% of C. glabrata biofilms. No synergistic effect was observed against C. krusei or C. tropicalis biofilms with any of the combinations. Rifampin or clarithromycin alone did not exert any effect on Candida species biofilms. Rifampin or clarithromycin combinations with amphotericin B might be of interest in the treatment of Candida biofilm-related infections.
在体外研究了两性霉素B单独使用或与利福平或克拉霉素联合使用对念珠菌生物膜的杀灭效果。两性霉素B的检测浓度为0.005至10mg/ml。利福平和克拉霉素的检测浓度为10mg/ml。我们研究了7株白色念珠菌、3株近平滑念珠菌、3株光滑念珠菌、3株克柔念珠菌和2株热带念珠菌。生物膜在96孔平底微量滴定板中培养48小时。两性霉素B与克拉霉素联合使用对66.6%的近平滑念珠菌、66.6%的光滑念珠菌和42.8%的白色念珠菌生物膜显示出协同作用。两性霉素B与利福平联合使用对66.6%的近平滑念珠菌、42.8%的白色念珠菌和33.3%的光滑念珠菌生物膜显示出协同作用。对于克柔念珠菌或热带念珠菌生物膜，任何联合用药均未观察到协同作用。利福平或克拉霉素单独使用对念珠菌生物膜没有任何作用。利福平或克拉霉素与两性霉素B联合使用可能对治疗念珠菌生物膜相关感染有意义。
