Gómez Carrillo M, Piccardo C, Libonatti O
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1992 Apr-Jun;24(2):91-101.
The main goal of the present paper was to analyze the molecular diversity of the principal neutralizing domain (V3 loop) of the HIV 1 gp120 in samples from patients of Argentina. The study was carried out on a total of 30 HIV 1 positive blood samples, obtained during 1991-1992, belonging to 15 intravenous drug users (group A), 5 homosexual men (group B), 8 children born to HIV 1 positive mothers (group C) and 2 AIDS patients (group D). By using extracted DNA from peripheral blood lymphocytes and from infected cells of the viral isolates in the case of the 2 AIDS patients, the V3 loop region was amplified by means of polymerase chain reaction. Direct sequencing by Sanger methodology was then performed on DNA fragments and nucleotide sequences obtained were translated into the correspondent amino acids. Consensus sequences for each group and a general consensus sequence were established (Table 1). Its alignment with V3 loop amino acid sequences of the major HIV 1 strains isolated worldwide is showed in table 2. Homology analysis between each sequence of the study population and sequence of different HIV 1 isolates showed that most of these samples share high homology with SF2 and BH10 strains. In contrast a low homology was found with JH3 and MN isolated (table 3). The presence of highly conserved amino acid residues as substitutions and insertions was determined in the Argentinian V3 loop sequences giving them a local pattern. The present paper is of great importance for our country, considering that the V3 loop is the main neutralizing domain becoming a major target in the development of HIV 1 vaccine. To our knowledge, this is the first report on the sequencing of the principal neutralizing domain of the Human Immunodeficiency Virus type 1 in Latin America.
本文的主要目的是分析阿根廷患者样本中HIV-1 gp120主要中和结构域（V3环）的分子多样性。该研究共对1991年至1992年期间获得的30份HIV-1阳性血样进行，这些血样分别来自15名静脉吸毒者（A组）、5名男同性恋者（B组）、8名HIV-1阳性母亲所生儿童（C组）和2名艾滋病患者（D组）。对于2名艾滋病患者，通过提取外周血淋巴细胞和病毒分离株感染细胞中的DNA，利用聚合酶链反应扩增V3环区域。然后采用桑格测序法对DNA片段进行直接测序，并将获得的核苷酸序列翻译成相应的氨基酸。确定了每组的共有序列和一个通用共有序列（表1）。表2展示了其与全球分离出的主要HIV-1毒株的V3环氨基酸序列的比对情况。研究人群的每个序列与不同HIV-1分离株序列之间的同源性分析表明，这些样本中的大多数与SF2和BH10毒株具有高度同源性。相比之下，与JH3和MN分离株的同源性较低（表3）。在阿根廷的V3环序列中确定了作为替换和插入的高度保守氨基酸残基的存在，赋予它们一种局部模式。考虑到V3环是主要的中和结构域，成为HIV-1疫苗开发的主要靶点，本文对我国具有重要意义。据我们所知，这是拉丁美洲关于人类免疫缺陷病毒1型主要中和结构域测序的首篇报道。
