使用单克隆抗体通过夹心酶联免疫吸附测定法检测人血清中的循环疟原虫抗原。

Detection of circulating plasmodial antigens in human sera by sandwich ELISA with monoclonal antibodies.

作者信息

Lim P K, Mak J W, Yong H S

机构信息

Malaria and Filariasis Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.

出版信息

Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):735-9.

PMID:1298082

Abstract

Two monoclonal antibodies (MAbs), one produced against Plasmodium falciparum (PF-IG8) and the other against P. cynomolgi (PC-IE12) schizont antigens were used in a sandwich ELISA for the detection of circulating plasmodial antigens in sera of patients infected with either P. falciparum, P. vivax or P. malariae. The mean +/- SD optical density (OD) values for the normal control group using PF-108 and PC-1E12 were 0.351 +/- 0.036 and 0.205 +/- 0.044, respectively. Mean OD values for the three infected groups were found to be significantly higher than those of the normal control group for both MAbs. However, ELISA values for individual serum specimens did not correlate with the level of parasitemia in the infected blood. Using a cut-off point of mean OD +/- 3 SD of the normal control group as indicating a positive reading, the specificity of this assay with both MAbs was 100%. The sensitivity of the assay using PF-1G8 was 95% while that obtained with PC-1E12 was 98%.

摘要

两种单克隆抗体（MAb），一种针对恶性疟原虫（PF-IG8）产生，另一种针对食蟹猴疟原虫（PC-IE12）裂殖体抗原产生，用于夹心酶联免疫吸附测定（ELISA），以检测感染恶性疟原虫、间日疟原虫或三日疟原虫患者血清中的循环疟原虫抗原。使用PF-108和PC-1E12时，正常对照组的平均±标准差光密度（OD）值分别为0.351±0.036和0.205±0.044。发现两个单克隆抗体的三个感染组的平均OD值均显著高于正常对照组。然而，单个血清标本的ELISA值与感染血液中的寄生虫血症水平无关。以正常对照组平均OD±3SD作为阳性读数的截断点，该检测方法对两种单克隆抗体的特异性均为100%。使用PF-1G8的检测方法的灵敏度为95%，而使用PC-1E12获得的灵敏度为98%。