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通过抑制酶联免疫吸附测定法检测血液中的疟疾抗原。

Detection of malaria antigen in blood by inhibition ELISA.

作者信息

Ray K, Parveen N, Srivaraman C A, Mukherjee A K, Raichoudhury A N

机构信息

Regional STD Teaching, Training & Research Centre, Safdarjang Hospital, New Delhi.

出版信息

J Commun Dis. 1992 Sep;24(3):138-44.

PMID:1344943
Abstract

Demonstration of parasite associated antigen in blood by inhibition ELISA in malaria patients and controls is described. The test was negative in all the healthy controls and positive in 90 per cent of the Plasmodium vivax malaria cases. The test was found to be quite sensitive, being able to detect 5 parasites/10(6) RBC in a case of natural P. falciparum infection. There was 95.3 per cent agreement between the results of this test and IgM-IIF test.

摘要

本文描述了通过抑制性酶联免疫吸附测定法(ELISA)检测疟疾患者和对照者血液中寄生虫相关抗原的情况。所有健康对照者的检测结果均为阴性,而90%的间日疟病例检测结果为阳性。该检测方法相当灵敏,在自然感染恶性疟原虫的病例中,能够检测到每10⁶个红细胞中有5个寄生虫。该检测结果与IgM间接免疫荧光试验(IgM-IIF)的结果之间的一致性为95.3%。

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