BAER B S, KRUEGER A P
J Gen Physiol. 1952 May;36(1):127-38. doi: 10.1085/jgp.36.1.127.
Experiments were performed to determine the mechanism of release of phage from the lysogenic strain of B. mycoides N. The results suggest that qualitatively the same situation obtains as in the phage-carrying cultures of B. megatherium 899 and E. coli Li; i.e., the population consists of two kinds of cells: "lysogènes potentiels" and "producteurs." Quantitatively, however, there are more "producers" in a broth culture of the lysogenic B. mycoides N, at least curing the first 4 to 8 hours after cells have been suspended in fresh medium, suggesting that the interaction between host and parasite is one in which the balance is easily swung in favor of the virus. These conclusions are based upon the following lines of evidence: (1) the slow "growth rate" of the lysogenic culture, (2) the fact that the colony count falls far below the plaque count or the filament count (which correspond) for a well washed suspension, (3) the increase in phage output in a large number of tubes, each containing a small number of lysogenic cells, after a few hours' incubation in nutrient broth at 30 degrees C.
进行了实验以确定噬菌体从蕈状芽孢杆菌N溶原性菌株中释放的机制。结果表明，定性地说，与巨大芽孢杆菌899和大肠杆菌Li的噬菌体携带培养物中的情况相同；即，群体由两种细胞组成：“潜在溶原菌”和“产生菌”。然而，定量地说，溶原性蕈状芽孢杆菌N的肉汤培养物中有更多的“产生菌”，至少在细胞悬浮于新鲜培养基后的最初4至8小时内是这样，这表明宿主与寄生物之间的相互作用是一种平衡很容易向有利于病毒的方向摆动的相互作用。这些结论基于以下几方面的证据：（1）溶原性培养物的缓慢“生长速率”，（2）菌落计数远低于经过充分洗涤的悬浮液的噬菌斑计数或丝状菌计数（二者相对应）这一事实，（3）在30℃的营养肉汤中孵育数小时后，大量每管含有少量溶原性细胞的试管中噬菌体产量的增加。
