控制巨大芽孢杆菌溶原培养物产生的因素。
Factors controlling the production of lysogenic cultures of B. megatherium.
作者信息
NORTHROP J H
出版信息
J Gen Physiol. 1961 May;44(5):859-67. doi: 10.1085/jgp.44.5.859.
(1) The proportion of infected B. megatherium cells which develop lysogenic colonies depends on the number and kind of infecting virus particles and on the culture medium in which the cells are growing. (2) Cells infected with 100 or more T virus particles (from megatherium 899) in yeast extract peptone disintegrate, produce very few virus particles, and less than one lysogenic colony per 10(7) infected cells. Cells infected with one or a few particles produce 500 to 1000 virus particles each and about 30 lysogenic colonies per 10(7) infected colonies. (3) T phage obtained from lysogenic magatherium KM cultures produces many more lysogenic cells than does the original megatherium 899 virus. (4) Cells infected with megatherium 899 T virus in peptone medium and then transferred to asparagine medium give rise to 10(6) lysogenic colonies per 10(7) infected cells and this transformation will occur even after the infected cells have been in peptone for 60 to 90 minutes and are beginning to produce virus particles. (5) Continued growth of KM strain with either C or T virus from megatherium 899 for several hundred generations in the steady state apparatus results in a lysogenic strain which produces several different types of virus.
(1) 形成溶源菌菌落的被感染巨大芽孢杆菌细胞的比例，取决于感染病毒粒子的数量和种类，以及细胞生长所在的培养基。(2) 在酵母提取物蛋白胨培养基中，被100个或更多T病毒粒子（来自巨大芽孢杆菌899）感染的细胞会解体，产生极少的病毒粒子，每10^7个被感染细胞产生不到一个溶源菌菌落。被一个或几个粒子感染的细胞每个产生500到1000个病毒粒子，每10^7个被感染菌落产生约30个溶源菌菌落。(3) 从溶源菌巨大芽孢杆菌KM培养物中获得的T噬菌体产生的溶源细胞比原始的巨大芽孢杆菌899病毒多得多。(4) 在蛋白胨培养基中被巨大芽孢杆菌899 T病毒感染，然后转移到天冬酰胺培养基中的细胞，每10^7个被感染细胞会产生10^6个溶源菌菌落，即使被感染细胞在蛋白胨中已培养60至90分钟并开始产生病毒粒子，这种转化仍会发生。(5) 在稳态装置中，KM菌株与来自巨大芽孢杆菌899的C或T病毒持续生长数百代，会产生一种能产生几种不同类型病毒的溶源菌株。
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