Wu Y H
Burn Department, Nan Fang Hospital, First Military College, GuangZhou.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1992 Sep;8(3):225-7, 250.
In this report, using the radio-ligand binding assay with [3H]-dexamethasone (Dex) as the ligand, we studied the changes in glucocorticoid receptor (GCR) in canine peripheral lymphocyte under unstressed condition and during the early postburn period (within 48 hours after burn). The results showed that the apparent binding capacity (Ro) and the apparent dissociation constant (Kd) of GCR were 8468 +/- 993 sites per cell and 10.2 +/- 0.6 nM (n = 6), respectively, under unstressed condition. The number of [3H]-Dex specific binding sites measured by the single saturated analysis (Rs) from 16 healthy mongrel dogs under unstressed condition was 4531 +/- 396 sites per cell. The Rs was decreased sharply at the second post-burn hour, reaching the lowest level of 2501 +/- 460 sites per cell. But it recovered to 78.9% of the level measured in unstressed condition. There was significant negative relationship between the plasma cortisol concentration and GCR levels of lymphocyte. The results suggest that the changes in GCR in postburn period may be related to the down-regulation of GCR by Gc; that GCR may be of very important significance in the pathophysiologic changes after burn injury.
在本报告中，我们以[3H] - 地塞米松（Dex）为配体，采用放射性配体结合分析法，研究了犬外周淋巴细胞在未应激状态下及烧伤后早期（烧伤后48小时内）糖皮质激素受体（GCR）的变化。结果显示，在未应激状态下，GCR的表观结合容量（Ro）和表观解离常数（Kd）分别为每个细胞8468±993个位点和10.2±0.6 nM（n = 6）。通过单次饱和分析（Rs）测定的16只健康杂种犬在未应激状态下的[3H] - Dex特异性结合位点数为每个细胞4531±396个位点。Rs在烧伤后第2小时急剧下降，降至每个细胞2501±460个位点的最低水平。但随后恢复到未应激状态下测定水平的78.9%。血浆皮质醇浓度与淋巴细胞GCR水平之间存在显著负相关。结果表明，烧伤后GCR的变化可能与糖皮质激素（Gc）对GCR的下调有关；GCR在烧伤后损伤的病理生理变化中可能具有非常重要的意义。
