Zhao Y, Liu Z M, Zhai C H
Zhonghua Nei Ke Za Zhi. 1989 Aug;28(8):479-81, 510.
The maximal binding capacity (Ro) and dissociation constant (Kd) of glucocorticoid receptor (GCR) in peripheral leukocytes were estimated by radioligand binding method and the plasma cortisol levels measured in 25 stroke patients (hemorrhagic 15, ischemic 10), compared with 12 healthy controls, the plasma cortisol level in stroke patients were significantly higher. The plasma cortisol level of 15 hemorrhagic and 10 ischemic stroke patients and 12 controls determined by RIA were 1.12 +/- 0.21, 0.90 +/- 0.28 and 0.45 +/- 0.09 nmol/L respectively. Ro of leukocytic GCR in hemorrhagic stroke were significantly lower than that of ischemic stroke and controls. No difference was found between ischemic stroke and controls. No difference was found between ischemic stroke and controls. Ro of GCR in 15 hemorrhagic stroke, 10 ischemic stroke patients and 12 controls were 3496 +/- 424, 5678 +/- 1101 and 5940 +/- 763 sites/cell respectively kd of GCR were 10.47 +/- 4.17, 7.01 +/- 3.24 and 5.81 +/- 1.05 nmol/L respectively. Affinity of GCR in 15 hemorrhagic stroke patients was significantly lower. In conclusion, lower number and affinity of leukocytic GCR and higher plasma cortisol level were demonstrated in hemorrhagic stroke patients, but normal number and affinity of GCR and higher plasma cortisol level in ischemic stroke patients.
采用放射配体结合法测定25例脑卒中患者（脑出血15例，脑缺血10例）外周血白细胞中糖皮质激素受体（GCR）的最大结合容量（Ro）和解离常数（Kd），并检测其血浆皮质醇水平，与12例健康对照者比较，脑卒中患者血浆皮质醇水平显著升高。采用放射免疫分析法测定15例脑出血、10例脑缺血脑卒中患者及12例对照者的血浆皮质醇水平分别为1.12±0.21、0.90±0.28和0.45±0.09nmol/L。脑出血组白细胞GCR的Ro显著低于脑缺血组和对照组。脑缺血组与对照组之间差异无统计学意义。15例脑出血、10例脑缺血脑卒中患者及12例对照者GCR的Ro分别为3496±424、5678±1101和5940±763位点/细胞，Kd分别为10.47±4.17、7.01±3.24和5.81±1.05nmol/L。15例脑出血患者GCR的亲和力显著降低。综上所述，脑出血患者白细胞GCR数量和亲和力降低，血浆皮质醇水平升高；而脑缺血患者GCR数量和亲和力正常，但血浆皮质醇水平升高。
