Kovács P, Hegyesi H, Csaba G
Department of Biology Semmelweis University of Medicine, Budapest, Hungary.
Cell Biochem Funct. 1992 Mar;10(1):31-4. doi: 10.1002/cbf.290100106.
Na-metavanadate and ouabain that act on Na+K(+)-ATPase had no influence on insulin binding to Tetrahymena immediately after treatment, but after 24 h considerably enhanced the binding capacity of generations of progeny. The increase in binding was of a similar magnitude to that elicited by insulin imprinting. Vanadate failed to increase the imprinting potential of insulin while ouabain even prevented insulin imprinting when administered together with insulin, but, did not affect imprinting when administered after insulin. By analogy with higher organisms it appears that inhibition of Na+K(+)-ATPase plays no role in the insulin-like effect of vanadate on the unicellular Tetrahymena, as judged also from the capacity to bind insulin of the generations of offspring.
作用于Na+K(+)-ATP酶的偏钒酸钠和哇巴因在处理后即刻对胰岛素与四膜虫的结合没有影响，但在24小时后显著增强了子代的结合能力。结合能力的增加幅度与胰岛素印记所引发的相似。钒酸盐未能增加胰岛素的印记潜力，而哇巴因在与胰岛素一起给药时甚至会阻止胰岛素印记，但在胰岛素给药后给药则不影响印记。与高等生物类似，从子代结合胰岛素的能力判断，Na+K(+)-ATP酶的抑制似乎在钒酸盐对单细胞四膜虫的胰岛素样作用中不起作用。
