Huang W M, Yan H, Jin J M, Yu C, Zhang H
Cardiovascular Research Laboratory, Xu Hui District Central Hospital, Shanghai.
Chin Med J (Engl). 1992 Dec;105(12):1014-9.
In 18 dogs ischemic left ventricular failure characterized by a 30 percent reduction in peak rate of rise of left ventricular pressure (+dp/dt) and elevation of left ventricular end-diastolic pressure (LVEDP) to 15 mmHg or more was produced by ligation of the proximal left anterior descending coronary artery followed by serial occlusions of the distal left circumflex coronary artery. In 10 days, administration of berberine in an intravenous bolus injection (1 mg/kg, within 3 minutes) followed by a constant infusion (0.2 mg/kg/min, 30 minutes) increased the cardiac output (CO) from 1.25 +/- 0.12 to 1.61 +/- 0.17 L/min (P < 0.05), and +dp/dt from 810 +/- 85 to 1021 +/- 130 mmHg/s (P < 0.01), and decreased LVEDP from 16.5 +/- 1.3 to 12.0 +/- 1.0 mmHg (P < 0.05), diastolic blood pressure from 94 +/- 6 to 84 +/- 5 mmHg (P < 0.01), systemic vascular resistance from 7303 +/- 278 to 5442 +/- 231 dynes.x/cm5 (P < 0.01), but did not affect the heart rate. Injection of 5% glucose with the same volume did not improve CO and dp/dt (P > 0.05) but increased the LVEDP from 17.1 +/- 1.4 to 17.8 +/- 1.6 mmHg (P < 0.01) in 8 dogs. The levels of plasma concentration of berberine was determined with high-performance liquid chromatography. The changes in plasma drug level were found parallel to hemodynamic effects of berberine. The results of this study showed that berberine was able to improve the impaired left ventricular function by its positive inotropic effect and mild systemic vasodilatation.
通过结扎左冠状动脉前降支近端，随后连续闭塞左旋支冠状动脉远端，在18只犬中制造出缺血性左心室衰竭模型，其特征为左心室压力上升最大速率（+dp/dt）降低30%，左心室舒张末期压力（LVEDP）升高至15 mmHg或更高。在10天内，静脉推注黄连素（1 mg/kg，3分钟内），随后持续输注（0.2 mg/kg/min，30分钟），使心输出量（CO）从1.25±0.12增加至1.61±0.17 L/min（P<0.05），+dp/dt从810±85增加至1021±130 mmHg/s（P<0.01），LVEDP从16.5±1.3降低至12.0±1.0 mmHg（P<0.05），舒张压从94±6降低至84±5 mmHg（P<0.01），全身血管阻力从7303±278降低至5442±231 dynes·s/cm5（P<0.01），但对心率无影响。给8只犬注射相同体积的5%葡萄糖，未改善CO和dp/dt（P>0.05），但使LVEDP从17.1±1.4升高至17.8±1.6 mmHg（P<0.01）。采用高效液相色谱法测定血浆黄连素浓度。发现血浆药物水平的变化与黄连素的血流动力学效应平行。本研究结果表明，黄连素能够通过其正性肌力作用和轻度全身血管舒张作用改善受损的左心室功能。
