酿酒酵母中紫外线诱导的含胸腺嘧啶二聚体的改良检测方法。
An improved assay of UV-induced thymine-containing dimers in Saccharomyces cerevisiae.
作者信息
Fäth W W, Brendel M
出版信息
Z Naturforsch C Biosci. 1975 Nov-Dec;30(6):804-10. doi: 10.1515/znc-1975-11-1220.
PMID:130013
Abstract
The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5'-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.
摘要
酵母中含胸腺嘧啶二聚体的检测方法基于高效的（[3H]-脱氧胸苷-5'-单磷酸）DNA特异性标记，并采用上行薄层色谱法。该方法能对低至500尔格/平方毫米的紫外线剂量进行令人满意的定量分析。
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