小麦胚细胞核的分离及其糖酵解代谢的某些方面。
The isolation of wheat germ nuclei and some aspects of their glycolytic metabolism.
作者信息
STERN H, MIRSKY A E
出版信息
J Gen Physiol. 1952 Nov;36(2):181-200. doi: 10.1085/jgp.36.2.181.
Abstract
- A procedure for isolating nuclei of the wheat germ in non-aqueous media has been described. 2. Such nuclei were shown to constitute about 50 per cent of the protoplasmic mass and to have a ribonucleic acid content of an order equivalent to that of the cytoplasm. 3. Studies of the distribution of the enzymes-aldolase, phosphoglyceraldehyde dehydrogenase, enolase, and pyruvate kinase-have revealed that the nuclei are the most vigorous sites of glycolytic activity. 4. Analysis of the DPN content of the nuclei in calf tissues-liver, pancreas, and heart-pointed to the probability that glycolytic activity is a characteristic common to many nuclei. 5. The significance of glycolytic activity to nuclear function has been discussed and some suggestive comparisons made between the two energy-yielding systems of glycolytic and oxidative phosphorylation.
摘要
- 已经描述了一种在非水介质中分离小麦胚芽细胞核的方法。2. 这种细胞核约占原生质质量的50%，其核糖核酸含量与细胞质相当。3. 对醛缩酶、磷酸甘油醛脱氢酶、烯醇化酶和丙酮酸激酶等酶分布的研究表明，细胞核是糖酵解活性最活跃的部位。4. 对小牛肝脏、胰腺和心脏组织细胞核中DPN含量的分析表明，糖酵解活性可能是许多细胞核共有的特征。5. 已经讨论了糖酵解活性对核功能的意义，并对糖酵解和氧化磷酸化这两种能量产生系统进行了一些有启发性的比较。
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