从活细胞中体外生产淋巴细胞和粒细胞增殖抑制剂（抑素？）

In vitro production of lymphocyte and granulocyte proliferation inhibitors (chalones?) from living cells.

作者信息

Maiolo A T, Cazzaniga E, Cortelezzi A, De Pangher V, Foa P, Lombardi L, Mozzana R, Polli E E

出版信息

Boll Ist Sieroter Milan. 1975 Jul 31;54(3):235-43.

Abstract

Media conditioned with viable rat and calf spleen lymphocytes were seen to be able to inhibit 3H-thymidine uptake by rat thymocytes and the mitogenic response of human lymphocytes to PHA but not bone marrow cells proliferation. The inhibitor appeared to have a molecular weight greater than 50,000 daltons, as determined by Amicon Diaflo ultrafiltration. Media conditioned with rat granulocytes had a similar tissue specific but species non-specific inhibitory action. Also human leukemic cells appeared able to produce in vitro an inhibitor of cell proliferation when a certain degree of differentiation was present.

摘要

用活的大鼠和小牛脾淋巴细胞处理过的培养基能够抑制大鼠胸腺细胞对³H-胸腺嘧啶核苷的摄取以及人淋巴细胞对PHA的促有丝分裂反应，但不能抑制骨髓细胞的增殖。通过Amicon Diaflo超滤测定，该抑制剂的分子量似乎大于50,000道尔顿。用大鼠粒细胞处理过的培养基具有类似的组织特异性但物种非特异性抑制作用。此外，当存在一定程度的分化时，人白血病细胞似乎能够在体外产生一种细胞增殖抑制剂。