Hiestand P C, Borel J F, Bauer W, Kis Z L, Magnée C, Stähelin H
Agents Actions. 1977 Sep;7(3):327-35. doi: 10.1007/BF01969564.
Aqueous extracts of lymphoid organs were prepared and fractionated by means of gel filtration, ion exchange chromatography, and isoelectric focusing. A protein-containing fraction with a molecular weight of approximately 80,000--90,000 and isoelectric points of 7.6 and 5.3--6.2 was isolated and shown to inhibit reproducibly both thymidine incorporation and proliferation of concanavalin A-stimulated mouse spleen lymphocytes in vitro. This effect appeared specific since proliferation of mastocytoma P-815 and leukemia L-1210 cells remained unaffected. A small molecular weight fraction (500 to 10,000 daltons) was also found to inhibit lymphocyte proliferation in vitro but was without apparent specificity for cell type.
制备了淋巴器官的水提取物，并通过凝胶过滤、离子交换色谱和等电聚焦进行分级分离。分离出一种分子量约为80,000 - 90,000、等电点为7.6和5.3 - 6.2的含蛋白质级分，结果表明其在体外可重复性地抑制胸苷掺入以及伴刀豆球蛋白A刺激的小鼠脾淋巴细胞的增殖。由于肥大细胞瘤P - 815和白血病L - 1210细胞的增殖未受影响，所以这种作用似乎具有特异性。还发现一种小分子量级分（500至10,000道尔顿）在体外可抑制淋巴细胞增殖，但对细胞类型没有明显的特异性。
