Sinichkina N A, Kravtsov A L, Naumov A V, Kuz'michenko I A, Taranenko T M, Zadumina S Iu
Zh Mikrobiol Epidemiol Immunobiol. 1992(11-12):52-4.
The influence of Y. pestis phospholipase D on the physiological state of leukocytes in the blood of guinea pigs was studied in vivo by flow impulse fluorometry with the use of fluorochrome acridine orange. During the first hours of observation the intensity of leukocyte fluorescence increased due to a rise in the number of polymorphonuclear leukocytes and changes in the permeability of cell membranes. Further changes in the intensity of the fluorescence of the material under study after 24 hours of observation occurred due to the appearance of activated lymphocytes in the blood stream. The processes normalized by day 21. The reaction of blood leukocytes to phospholipase D was specific in comparison with the reaction to capsular antigen, "mouse" toxin, lipopolysaccharide and the main somatic antigen.
利用荧光染料吖啶橙，通过流动脉冲荧光测定法在体内研究了鼠疫耶尔森菌磷脂酶D对豚鼠血液中白细胞生理状态的影响。在观察的最初几个小时内，由于多形核白细胞数量的增加和细胞膜通透性的变化，白细胞荧光强度增加。观察24小时后，由于血流中出现活化淋巴细胞，所研究物质的荧光强度进一步发生变化。到第21天这些过程恢复正常。与对荚膜抗原、“鼠”毒素、脂多糖和主要菌体抗原的反应相比，血液白细胞对磷脂酶D的反应具有特异性。
