[缺氧及随后的气压给氧对大鼠肝脏微粒体氧化系统功能的影响]

[The effect of hypoxia and of subsequent baro-oxygenation on the function of the microsomal oxidation system in the rat liver].

作者信息

Shchugaleĭ V S, Ananian A A, Miliutina N P, Sadekova S I

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1992(7):86-94.

Abstract

The increase of cytochrome P-450 by 34% and its catalytic activity with substrate amidopyrine by 57% as compared with control has been shown under hypoxia (0.029 MPa, 1 h). Hyperoxia (0.2 MPa, 1 h) increases the metabolism of amidopyrine by 148%, benzo[a]pyrene by 158% and aniline by 114% and consecutive affection of hypoxia and hyperoxia--by 247, 45 and 138% correspondingly at fixed cytochrome P-450 amount in both series. The amount of diene conjugates and Schiff's bases under hypoxia increases by 40 and 69% correspondingly, the activity of SOD and catalase decreases by 25 and 23%. The activity of hyperoxia raises the diene conjugate content by 19% at all this SOD activity increases by 95%. Consecutive affection of hypoxia and hyperoxia increases the level of diene conjugates and Schiff's bases by 26 and 23% correspondingly, without changing SOD and catalase activity. The relative microsomal viscosity of lipid layer and zones of enzyme-lipid contacts decreased by 20 and 24% under hypoxia, but under hyperoxia and consecutive affection and hypoxia and hyperoxia it increases by 29-28% and 56-40% correspondingly.

摘要

与对照组相比，在低氧（0.029兆帕，1小时）条件下，细胞色素P - 450增加了34%，其对底物氨基比林的催化活性增加了57%。高氧（0.2兆帕，1小时）使氨基比林的代谢增加了148%，苯并[a]芘增加了158%，苯胺增加了114%；在两个系列中细胞色素P - 450量固定的情况下，低氧和高氧的连续作用分别使代谢增加了247%、45%和138%。在低氧条件下，二烯共轭物和席夫碱的量分别增加了40%和69%，超氧化物歧化酶（SOD）和过氧化氢酶的活性分别降低了25%和23%。高氧作用使二烯共轭物含量增加了19%，同时SOD活性增加了95%。低氧和高氧的连续作用使二烯共轭物和席夫碱的水平分别增加了26%和23%，而SOD和过氧化氢酶的活性没有变化。在低氧条件下，脂质层和酶 - 脂质接触区域的相对微粒体粘度降低了20%和24%，但在高氧、低氧和高氧的连续作用下，其分别增加了29% - 28%和56% - 40%。