Boorsma D M, Nieboer C, Kalsbeek G L
J Cutan Pathol. 1975;2(6):294-301. doi: 10.1111/j.1600-0560.1975.tb00181.x.
Two immunohistochemical methods, using the enzyme horseradish peroxidase, the direct immunoperoxidase (DIP) and the immunoglobulin-enzyme bridge (IEB) method were applied on 129 skin specimens of 81 patients with lupus erythematosus, bullous pemphigoid, pemphigus vulgaris and rosacea. These methods were compared with each other and with the immunofluorescence (IF) method. The DIP method was preferred to the IEB method because of the greater contrast between the specific staining and the nonspecific staining of the former. The results obtained with both peroxidase methods were comparable with those of the IF method.
采用两种免疫组织化学方法，即使用辣根过氧化物酶的直接免疫过氧化物酶法（DIP）和免疫球蛋白 - 酶桥法（IEB），对81例红斑狼疮、大疱性类天疱疮、寻常型天疱疮和酒渣鼻患者的129份皮肤标本进行检测。将这些方法相互比较，并与免疫荧光法（IF）进行比较。由于DIP法的特异性染色与非特异性染色之间对比度更高，因此比IEB法更受青睐。两种过氧化物酶法所获得的结果与免疫荧光法的结果相当。
