Tandon A, Agarwal S K, Saha K
Maulana Azad Medical College, New Delhi, India.
Natl Med J India. 1992 Jul-Aug;5(4):166-8.
Cellular immunity may play a major role in the pathogenesis of amoebic liver abscess but there is little data on the effect of treatment on T cell subpopulations in such patients.
We performed a prospective, controlled study of the T lymphocyte subpopulations in 17 patients with amoebic liver abscess before, and at 4 and 8 weeks after treatment with metronidazole (30 mg/kg/day). T4 and T8 cells were studied using monoclonal antibodies by the alkaline phosphatase anti-alkaline phosphatase staining technique.
The mean T4 cell percentages in the acute stage of illness and at 4 and 8 weeks after treatment were 27, 26 and 27 respectively and the mean T8 cell percentages were 19, 24 and 29. The T4:T8 ratio at the acute stage was 1.7, and 1.1 and 1.2 at 4 and 8 weeks of therapy. The T4:T8 ratio at the acute stage did not differ significantly (p > 0.05) from that in the control group. However, at 4 and 8 weeks after therapy there was a significant increase (p < 0.05) in the T8 cells with no significant change in the T4 cells.
We suggest that sensitization of the T8 cells occurs in patients with amoebic liver abscess in the later phase of the disease. This may be responsible for the elimination of the parasite from the human host.
细胞免疫可能在阿米巴肝脓肿的发病机制中起主要作用，但关于治疗对此类患者T细胞亚群的影响的数据很少。
我们对17例阿米巴肝脓肿患者在使用甲硝唑（30mg/kg/天）治疗前、治疗后4周和8周时的T淋巴细胞亚群进行了一项前瞻性对照研究。使用单克隆抗体通过碱性磷酸酶抗碱性磷酸酶染色技术研究T4和T8细胞。
疾病急性期以及治疗后4周和8周时的平均T4细胞百分比分别为27、26和27，平均T8细胞百分比分别为19、24和29。急性期的T4:T8比值为1.7，治疗4周和8周时分别为1.1和1.2。急性期的T4:T8比值与对照组相比无显著差异（p>0.05）。然而，治疗后4周和8周时T8细胞显著增加（p<0.05），而T4细胞无显著变化。
我们认为，在疾病后期，阿米巴肝脓肿患者的T8细胞会发生致敏。这可能是寄生虫从人体宿主中被清除的原因。
