Blanco F, Kalsi J, Ravirajan C T, Speight P, Bradwell A R, Isenberg D A
Department of Rheumatology Research, University College and Middlesex School of Medicine, London, UK.
Lupus. 1992 Dec;1(6):391-9. doi: 10.1177/096120339200100609.
In this study the concentration of the different subclasses of IgG in sera from patients with a range of autoimmune rheumatic diseases (ARD) was detected by radial immunodiffusion. In the second part the IgG subclasses of autoantibodies that recognize single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), Ro, La, Sm and RNP in patients with ARD were measured by enzyme-linked immunosorbent assay. We studied 15 patients with lupus, 20 patients each with primary and secondary Sjögren's syndrome (SS) and 10 each with rheumatoid arthritis (RA), scleroderma and myositis. Twenty healthy controls were also measured. The serum concentration of IgG2 in ARD patients was generally reduced. In contrast, the concentrations of IgG1, IgG3 and IgG4 subclasses were normal or raised. A high degree of correspondence in the IgG1, IgG2 and IgG3 responses to dsDNA and ssDNA in SLE was found. Notable differences in the IgG1 anti-Ro and ssDNA responses compared to the other subclasses were seen in 1 degree and 2 degrees SS. In addition, an unexpected high level of IgG4 antibodies to ssDNA in 1 degree SS (65%) and IgG4 antibodies to Sm/RNP in RA was observed.
在本研究中，通过放射免疫扩散法检测了一系列自身免疫性风湿性疾病（ARD）患者血清中不同IgG亚类的浓度。在第二部分中，采用酶联免疫吸附测定法检测了ARD患者中识别单链DNA（ssDNA）、双链DNA（dsDNA）、Ro、La、Sm和RNP的自身抗体的IgG亚类。我们研究了15例狼疮患者、20例原发性和继发性干燥综合征（SS）患者以及10例类风湿关节炎（RA）、硬皮病和肌炎患者。还检测了20名健康对照者。ARD患者中IgG2的血清浓度普遍降低。相比之下，IgG1、IgG3和IgG4亚类的浓度正常或升高。在系统性红斑狼疮（SLE）中，发现IgG1、IgG2和IgG3对dsDNA和ssDNA的反应高度一致。在原发性和继发性SS中，与其他亚类相比，IgG1抗Ro和抗ssDNA反应存在显著差异。此外，在原发性SS中观察到意外的高比例（65%）的抗ssDNA IgG4抗体，在RA中观察到抗Sm/RNP IgG4抗体。
