Tanaka H, Yamanouchi M, Imai S, Hayashi Y
Division of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1992 Dec;38(6):545-54. doi: 10.3177/jnsv.38.545.
The effects of prenatal triethylene tetramine dihydrochloride (Trien-2HCl) exposure on fetal mice have been investigated on gestational day 19. Trien-2HCl was given throughout pregnancy at levels of 0 (control), 3,000, 6,000, or 12,000 ppm as drinking water, ad libitum. At the level of 12,000 ppm, the frequency of total resorption tended to be high and that of fetal viability tended to be low, as compared to controls. Decreased maternal weight was observed in body, but not in liver, at the level of 12,000 ppm. Fetal body and cerebrum weights significantly decreased at the levels of 6,000 and 12,000 ppm; however, fetal liver weight remained unchanged. Maternal serum copper concentration was not affected by the Trien-2HCl. Fetal copper concentrations of liver and cerebrum were significantly lower in the Trien-2HCl-treated groups than in the controls, with levels decreasing in a dose-related manner. When the copper and zinc concentrations in the group treated at 12,000 ppm were compared with those in controls, significant decreases in both metals were observed in placenta but not in maternal liver. Changes in fetal zinc concentration varied by tissues: i.e., an increase in liver and no change in cerebrum. Fetal abnormalities were frequently observed in brain, and the frequency was increased with increasing levels of the Trien-2HCl. These results suggest that fetal brain abnormalities caused by Trien-2HCl may be due in part to induction of copper deficiency, which is almost equivalent to that in brindled mutant mouse.
已在妊娠第19天研究了产前暴露于二盐酸三乙烯四胺（Trien-2HCl）对胎鼠的影响。在整个孕期，随意给予0（对照）、3000、6000或12000 ppm水平的Trien-2HCl作为饮用水。与对照组相比，在12000 ppm水平时，总吸收频率趋于升高，胎儿存活率趋于降低。在12000 ppm水平时，观察到母体体重减轻，但肝脏体重未减轻。在6000和12000 ppm水平时，胎儿体重和脑重显著降低；然而，胎儿肝脏重量保持不变。母体血清铜浓度不受Trien-2HCl的影响。与对照组相比，Trien-2HCl处理组的胎儿肝脏和脑铜浓度显著降低，且水平呈剂量相关下降。当将12000 ppm处理组的铜和锌浓度与对照组进行比较时，胎盘内两种金属均显著降低，但母体肝脏中未降低。胎儿锌浓度的变化因组织而异：即肝脏中增加而脑中无变化。在脑中经常观察到胎儿异常，且异常频率随Trien-2HCl水平的升高而增加。这些结果表明，Trien-2HCl引起的胎儿脑异常可能部分归因于铜缺乏的诱导，这与斑驳突变小鼠中的情况几乎相当。
