Reinstein N H, Lönnerdal B, Keen C L, Hurley L S
J Nutr. 1984 Jul;114(7):1266-79. doi: 10.1093/jn/114.7.1266.
The interaction between zinc and copper in the pregnant rat was investigated by feeding Sprague-Dawley rats, from mating to term, diets that varied in zinc and copper concentrations. A factorial design was used with diets containing 1, 10, 100 or 1000 micrograms zinc/gram and 0.5, 5, 10 or 100 micrograms copper/gram. Malformations were found only in fetuses from rats fed the zinc-deficient diets (1 microgram/g), and the frequency of malformed fetuses increased as concentration of dietary copper increased. Maternal and fetal tissues of rats fed diets deficient in either zinc or copper were significantly lower in these elements than were those of rats fed a control diet. An antagonistic effect of dietary zinc on maternal and fetal tissue copper concentration was observed. The concentration of fetal liver zinc from rats fed a copper-deficient diet was significantly higher than controls. The concentration of iron in maternal and fetal tissues was significantly affected by the level of dietary zinc, with values highest in the zinc-deficient groups. Maternal plasma cholesterol and triglyceride levels were significantly lower than normal in the zinc-deficient animals. These data show that when the concentration of zinc in the diet is deficient an interaction can occur between dietary zinc and copper such that high levels of copper potentiate the teratogenicity of zinc deficiency.
通过给妊娠的斯普拉格-道利大鼠从交配到足月喂食锌和铜浓度不同的日粮，研究了锌与铜在妊娠大鼠体内的相互作用。采用析因设计，日粮中锌含量为1、10、100或1000微克/克，铜含量为0.5、5、10或100微克/克。仅在喂食缺锌日粮（1微克/克）的大鼠所产胎儿中发现畸形，且畸形胎儿的频率随日粮铜浓度的增加而增加。喂食缺锌或缺铜日粮的大鼠的母体和胎儿组织中这些元素的含量明显低于喂食对照日粮的大鼠。观察到日粮锌对母体和胎儿组织铜浓度有拮抗作用。喂食缺铜日粮的大鼠胎儿肝脏锌浓度明显高于对照组。日粮锌水平显著影响母体和胎儿组织中的铁含量，缺锌组的值最高。缺锌动物的母体血浆胆固醇和甘油三酯水平明显低于正常水平。这些数据表明，当日粮锌浓度不足时，日粮锌与铜之间可能会发生相互作用，即高铜水平会增强锌缺乏的致畸性。
