Cosma G N, Toniolo P, Currie D, Pasternack B S, Garte S J
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
Cancer Epidemiol Biomarkers Prev. 1992 Jan-Feb;1(2):137-42.
We have conducted a pilot study to assess levels of cytochrome CYP1A1 gene expression in human peripheral lymphocytes as a molecular biomarker assay for polycyclic hydrocarbon exposure. Basal and 3-methylcholanthrene-induced levels of gene expression were measured by standard slot-blot mRNA analyses in mitogen-stimulated cultures of peripheral blood lymphocytes from creosote-exposed railroad workers and unexposed control subjects. Dermal and inhalation exposure of workers to creosote may vary substantially as a function of working conditions related to temperature. Therefore, blood specimens were collected from separate groups during the winter, fall, and summer. Basal and induced CYP1A1 gene expression levels were not elevated in workers from any of the three seasonal studies. However, induced/basal (inducibility) CYP1A1 mRNA ratios from workers sampled in the summer (when actual exposures were greatest) were significantly higher when compared to those of controls (P < 0.01). These studies demonstrate the potential usefulness of specific gene expression assays in human peripheral lymphocytes for the assessment of carcinogen exposure in human populations.
我们开展了一项初步研究，以评估人类外周血淋巴细胞中细胞色素CYP1A1基因的表达水平，作为多环烃暴露的分子生物标志物检测方法。通过标准狭缝印迹mRNA分析，在来自接触杂酚油的铁路工人和未接触杂酚油的对照受试者的外周血淋巴细胞丝裂原刺激培养物中，测量基础和3-甲基胆蒽诱导的基因表达水平。工人经皮肤和吸入接触杂酚油的情况，可能会因与温度相关的工作条件而有很大差异。因此，在冬季、秋季和夏季分别从不同组中采集血液样本。在这三项季节性研究中，任何一组工人的基础和诱导CYP1A1基因表达水平均未升高。然而，与对照组相比，夏季（实际暴露量最大时）采集样本的工人的诱导/基础（诱导性）CYP1A1 mRNA比值显著更高（P < 0.01）。这些研究表明，特定基因表达检测在人类外周血淋巴细胞中对于评估人群中的致癌物暴露具有潜在用途。
