Sun Yun-guang, Zhou Zhi-jun, Zhang Xue-mei, Gu Xi-an, Jin Tai-yi
School of Public Health, Fudan University, Shanghai 200032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Feb;22(1):3-6.
To study the expression of muscarinic receptor M(3) gene in peripheral blood lymphocytes of workers exposed to organophosphorus pesticides (OPPs) and to explore its role in the adverse effects of OPPs.
The lymphocytes of peripheral blood from 33 workers exposed to dimethoate and 15 control people were isolated and treated with saline and dimethoate respectively in vitro. RT-PCR technique was used to determine M(3) gene expression. Basal and inducible gene expression levels were measured.
There was no significant difference in basal gene expression level between exposed group and control group, while the inducible gene expression level was significantly higher in exposure group (1.92 +/- 1.07) than in control group (1.22 +/- 0.19) and basal level (1.49 +/- 0.45, P < 0.05). No differences in basal and inducible gene expression level were found between male and female people in both exposed and control group. The level of inducible M(3) gene expression increased with the increase in length of exposure time [< 5 a: (1.69 +/- 0.95), 5 - 25 a: (1.91 +/- 1.03), > 25 a: (2.09 +/- 1.25), the latter was significantly different from that of < 5 a (P < 0.05)].
After long-term exposure to OPPs, the basal M(3) receptor gene expression level in the exposed workers did not show any difference from the control group, but the inducible gene expression level (treated with dimethoate in vitro) was increased and related to the extent of exposure to dimethoate.
研究有机磷农药（OPPs）暴露工人外周血淋巴细胞中M3型毒蕈碱受体基因的表达情况，并探讨其在OPPs不良反应中的作用。
分离33名乐果暴露工人和15名对照人员的外周血淋巴细胞，分别在体外用生理盐水和乐果处理。采用逆转录-聚合酶链反应（RT-PCR）技术检测M3基因表达。测定基础和诱导基因表达水平。
暴露组与对照组基础基因表达水平无显著差异，但暴露组诱导基因表达水平（1.92±1.07）显著高于对照组（1.22±0.19）和基础水平（1.49±0.45，P<0.05）。暴露组和对照组男性与女性之间基础和诱导基因表达水平均无差异。诱导型M3基因表达水平随暴露时间延长而升高[<5年：（1.69±0.95），5-25年：（1.91±1.03），>25年：（2.09±1.25），后者与<5年者差异有统计学意义（P<0.05）]。
长期暴露于OPPs后，暴露工人的基础M3受体基因表达水平与对照组无差异，但体外经乐果处理后的诱导基因表达水平升高，且与乐果暴露程度相关。
