Love R R, Surawicz T S, Morrissey J F, Verma A K
Cancer Prevention Program, University of Wisconsin Clinical Cancer Center, Madison.
Cancer Epidemiol Biomarkers Prev. 1992 Mar-Apr;1(3):195-8.
Ornithine decarboxylase (ODC), a key enzyme in mammalian polyamine biosynthesis, has been proposed to be a marker of colonic epithelial cell proliferation and risk for colorectal cancer. We investigated the basal levels of ODC activity in sigmoid and rectal mucosae, and basal and tumor promoter 12-O-tetradecanoylphorbol-13-acetate-induced levels of skin ODC activity in individuals with a personal history of colon cancer (n = 9 colon; n = 58 skin), a family history of nonpolyposis hereditary colorectal cancer (n = 49; n = 42), adenomas (n = 16; n = 40), and healthy, family history-negative control subjects (n = 40; n = 79). Using a fresh tissue assay and samples obtained after a standard colon lavage preparation, colon mucosal ODC levels ranged from 0 to 192 pmol/mg/h (sigmoid, 0-163 pmol/mg/h; mean, 36 +/- 32 pmol/mg/h; rectum, 0-192 pmol/mg/h; mean, 35 +/- 32 pmol/mg/h). No differences among the four groups of subjects were found for either colon or skin ODC levels, and there were no sex differences overall or in any group. These results are not compatible with the suggestion that ODC levels are a useful marker of risk for colorectal cancer.
鸟氨酸脱羧酶（ODC）是哺乳动物多胺生物合成中的关键酶，有人提出它是结肠上皮细胞增殖和结直肠癌风险的标志物。我们研究了患有结肠癌个人史的个体（n = 9例结肠；n = 58例皮肤）、非息肉病性遗传性结直肠癌家族史的个体（n = 49例；n = 42例）、腺瘤患者（n = 16例；n = 40例）以及健康的、家族史阴性的对照受试者（n = 40例；n = 79例）的乙状结肠和直肠黏膜中ODC活性的基础水平，以及皮肤中基础和肿瘤启动子12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯诱导的ODC活性水平。使用新鲜组织检测法和标准结肠灌洗准备后获得的样本，结肠黏膜ODC水平范围为0至192 pmol/mg/h（乙状结肠，0至163 pmol/mg/h；平均值，36±32 pmol/mg/h；直肠，0至192 pmol/mg/h；平均值，35±32 pmol/mg/h）。在四组受试者中，结肠或皮肤ODC水平均未发现差异，总体及任何一组中均无性别差异。这些结果与ODC水平是结直肠癌风险有用标志物的观点不相符。
