Ohira T, Ohe Y, Saijo N
Pharmacology Division, National Cancer Center Institute, Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Dec;30 Suppl:48-51.
It is difficult to induce anti-tumor immunity in tumors with low antigenicity. In order to develop a more effective method of immunotherapy, we transfected interleukin-2 (IL-2), interleukin-4 (IL-4) and interleukin-6 (IL-6) genes into Lewis lung carcinoma (LLC) cells. Then, 1 x 10(6) LLC-IL-2, LLC-IL-4 or LLC-IL-6 cells were transplanted into C57BL/6 mice subcutaneously. All mice transplanted with LLC-IL2 and half those with LLC-IL-4 rejected the tumor cells. Survival time of LLC-IL-6 transplanted mice was significantly shorter than that of LLC transplanted mice, with no difference in tumor growth. These data suggest that transplantation of IL-2 or IL-4 gene transfected cells could effectively induce immunity against LLC. IL-6 transfection did not induce immunity, but induced cachexia.
在低抗原性肿瘤中诱导抗肿瘤免疫是困难的。为了开发一种更有效的免疫治疗方法，我们将白细胞介素-2（IL-2）、白细胞介素-4（IL-4）和白细胞介素-6（IL-6）基因转染到刘易斯肺癌（LLC）细胞中。然后，将1×10⁶个LLC-IL-2、LLC-IL-4或LLC-IL-6细胞皮下移植到C57BL/6小鼠体内。所有移植了LLC-IL2的小鼠以及一半移植了LLC-IL-4的小鼠排斥肿瘤细胞。移植了LLC-IL-6的小鼠的存活时间明显短于移植了LLC的小鼠，肿瘤生长情况无差异。这些数据表明，移植IL-2或IL-4基因转染的细胞可以有效诱导针对LLC的免疫。IL-6转染未诱导免疫，但诱导了恶病质。
