艰难梭菌毒素和变形链球菌葡糖基转移酶同源重复C端碳水化合物结合位点模块化结构的证据。
Evidence for a modular structure of the homologous repetitive C-terminal carbohydrate-binding sites of Clostridium difficile toxins and Streptococcus mutans glucosyltransferases.
作者信息
von Eichel-Streiber C, Sauerborn M, Kuramitsu H K
机构信息
Institut für Medizinische Mikrobiologie, Johannes-Gutenberg-Universität, Mainz, Federal Republic of Germany.
出版信息
J Bacteriol. 1992 Oct;174(20):6707-10. doi: 10.1128/jb.174.20.6707-6710.1992.
Abstract
The homologous C-terminal repeats of Clostridium difficile toxins (ToxA and ToxB) and streptococcal glucosyltransferases appear to mediate protein-carbohydrate interactions at cellular binding sites with sugar moieties as substrates. A consensus sequence of 134 repeating units from gram-positive bacteria indicates that these repeats have a modular design with (i) a stretch of aromatic amino acids proposed to be involved in the primary carbohydrate-protein interaction, (ii) an amplification of this interaction by repetition of the respective sequences, and (iii) a second domain, not characterized, that is responsible for carbohydrate specificity.
摘要
艰难梭菌毒素（ToxA和ToxB）与链球菌葡糖基转移酶的同源C末端重复序列似乎在细胞结合位点介导蛋白质与碳水化合物的相互作用，其中糖部分作为底物。来自革兰氏阳性菌的134个重复单元的共有序列表明，这些重复序列具有模块化设计，即（i）一段芳香族氨基酸，被认为参与了碳水化合物与蛋白质的主要相互作用；（ii）通过各自序列的重复来增强这种相互作用；（iii）第二个未明确特征的结构域，负责碳水化合物特异性。
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