从海洋酵母汉逊德巴利酵母中分离并鉴定自主复制序列（ARSD）

Isolation and characterization of an autonomously replicating sequence (ARSD) from the marine yeast Debaryomyces hansenii.

作者信息

Govind N S, Banaszak A T

机构信息

Marine Science Institute, University of California, Santa Barbara 93106.

出版信息

Mol Mar Biol Biotechnol. 1992 Jun;1(3):215-8.

PMID:1308205

Abstract

The marine yeast Debaryomyces hansenii is known to tolerate salinities ranging from 0 to 24%. As a first step toward the molecular analysis of halotolerance in this organism, we report the isolation of an autonomously replicating sequence (ARS) and its use in the construction of a shuttle vector. The ARS from D. hansenii (ARSD) is 0.4 kbp long, and the function rests in 0.13 kbp of the sequence. Sequence analysis of ARSD shows strong homology to ARS from other organisms, including a 12-bp consensus sequence common to all ARS functional in Saccharomyces cerevisiae.

摘要

已知海洋酵母汉逊德巴利酵母能够耐受0%至24%的盐度。作为对该生物体耐盐性进行分子分析的第一步，我们报告了一个自主复制序列（ARS）的分离及其在穿梭载体构建中的应用。来自汉逊德巴利酵母的ARS（ARSD）长0.4千碱基对，其功能存在于该序列的0.13千碱基对中。对ARSD的序列分析表明，它与其他生物体的ARS具有很强的同源性，包括酿酒酵母中所有功能性ARS共有的一个12碱基一致序列。