Carlson M, Håkansson L, Kämpe M, Stålenheim G, Peterson C, Venge P
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
J Allergy Clin Immunol. 1992 Jan;89(1 Pt 1):131-9. doi: 10.1016/s0091-6749(05)80050-8.
The secretion of granule proteins from eosinophils and neutrophils was studied in isolated cells, obtained from 11 pollen-atopic patients with asthma, twice during and twice outside pollen season. Granulocytes were stimulated with serum-opsonized Sephadex particles, and the released amount of eosinophil cationic protein (ECP), eosinophil protein X (EPX), and myeloperoxidase (MPO) were measured by means of specific radioimmunoassay (RIA). Eosinophils from the pollen-atopic patients obtained during pollen season released significantly more (p less than 0.02) ECP and EPX than cells from the same patients obtained before pollen season. The released amount of ECP and EPX was correlated (r = 0.54; p less than 0.003) to the total pollen count. The release of MPO from neutrophils was only raised (p less than 0.01) at the end of the pollen season. Serum concentrations of ECP and EPX and blood eosinophil counts were significantly raised (p less than 0.002, p less than 0.001, and p less than 0.009, respectively) before pollen season and increased further at the end of the pollen season. There were no changes in lung function during pollen season and consequently no discernible relationships to eosinophil and neutrophil degranulation. We conclude that eosinophils and, to some extent, neutrophils from birch pollen-atopic subjects have an increased propensity to secrete their granule proteins during a pollen season. We suggest that these cells have been primed as a consequence of allergen exposure.
对11名花粉过敏型哮喘患者在花粉季节期间两次、花粉季节之外两次采集的分离细胞中嗜酸性粒细胞和中性粒细胞的颗粒蛋白分泌情况进行了研究。用血清调理的葡聚糖凝胶颗粒刺激粒细胞，通过特异性放射免疫测定法（RIA）测量嗜酸性粒细胞阳离子蛋白（ECP）、嗜酸性粒细胞蛋白X（EPX）和髓过氧化物酶（MPO）的释放量。花粉季节采集的花粉过敏患者的嗜酸性粒细胞释放的ECP和EPX明显多于（p<0.02）花粉季节之前采集的同一患者的细胞。ECP和EPX的释放量与总花粉计数相关（r = 0.54；p<0.003）。中性粒细胞MPO的释放仅在花粉季节末期升高（p<0.01）。花粉季节之前，血清中ECP和EPX的浓度以及血液嗜酸性粒细胞计数显著升高（分别为p<0.002、p<0.001和p<0.009），在花粉季节末期进一步升高。花粉季节期间肺功能没有变化，因此与嗜酸性粒细胞和中性粒细胞脱颗粒没有明显关系。我们得出结论，桦树花粉过敏受试者的嗜酸性粒细胞以及在一定程度上中性粒细胞在花粉季节分泌其颗粒蛋白的倾向增加。我们认为这些细胞因接触过敏原而被致敏。
