Carlson M, Peterson C, Venge P
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Allergy. 1993 Aug;48(6):437-42.
The priming effect of interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte/macrophage-colony stimulating factor (GM-CSF) on eosinophil and neutrophil degranulation was studied. Granulocytes were obtained from normal donors, and degranulation was induced by incubation with serum-opsonized Sephadex particles. The released amounts of eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), and lactoferrin (LF) were measured by radioimmunoassay (RIA). The effect of IL-5 was dose- and time-dependent, with a maximal enhancement of ECP and EPX release of 71% (P < 0.03) and 66% (P < 0.03), respectively. Neutrophil degranulation, however, was unaffected. IL-3 was marginally effective, whereas GM-CSF seemed to act as a secretagogue for both eosinophil and neutrophil degranulation. We conclude that IL-5 selectively primes eosinophil degranulation, whereas IL-3 and GM-CSF seem to act as secretagogues for eosinophils and neutrophils. The results indicate that IL-5 may be involved in the priming of eosinophils as observed in patients with asthma and hypereosinophilic syndrome (HES).
研究了白细胞介素-3（IL-3）、白细胞介素-5（IL-5）和粒细胞/巨噬细胞集落刺激因子（GM-CSF）对嗜酸性粒细胞和中性粒细胞脱颗粒的启动作用。粒细胞取自正常供体，通过与血清调理的葡聚糖凝胶颗粒孵育诱导脱颗粒。采用放射免疫分析法（RIA）测定嗜酸性粒细胞阳离子蛋白（ECP）、嗜酸性粒细胞蛋白X（EPX）、髓过氧化物酶（MPO）和乳铁蛋白（LF）的释放量。IL-5的作用呈剂量和时间依赖性，ECP和EPX释放的最大增强分别为71%（P<0.03）和66%（P<0.03）。然而，中性粒细胞脱颗粒未受影响。IL-3的作用微乎其微，而GM-CSF似乎是嗜酸性粒细胞和中性粒细胞脱颗粒的促分泌剂。我们得出结论，IL-5选择性地启动嗜酸性粒细胞脱颗粒，而IL-3和GM-CSF似乎是嗜酸性粒细胞和中性粒细胞的促分泌剂。结果表明，IL-5可能参与了哮喘和高嗜酸性粒细胞综合征（HES）患者中观察到的嗜酸性粒细胞启动过程。
