Carpenter S, Miller L D, Alexandersen S, Whetstone C A, VanDerMaaten M J, Viuff B, Wannemuehler Y, Miller J M, Roth J A
Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011.
J Virol. 1992 Feb;66(2):1074-83. doi: 10.1128/JVI.66.2.1074-1083.1992.
The early pathogenic effects of bovine immunodeficiency-like virus (BIV) were studied in calves experimentally inoculated with BIV. All animals inoculated with BIV R29-infected cells seroconverted by 6 weeks postinoculation, and BIV was recoverable from each animal at 2 weeks postinoculation. However, levels of BIV replication in vivo appeared to be low. In situ hybridization studies indicated that during peak periods of viral replication in vivo, less than 0.03% of peripheral blood mononuclear cells were expressing detectable levels of viral RNA. Moreover, the levels of viral RNA in these cells in vivo were less than 1/10 the levels observed in persistently infected cells in vitro. BIV-inoculated calves had significantly higher numbers of circulating lymphocytes, and follicular hyperplasia was observed in lymph nodes, hemal nodes, and spleen. The histopathological changes observed in BIV-infected calves were similar to changes found early after infection with the immunosuppressive lentiviruses, including human immunodeficiency virus type 1.
在通过实验接种牛免疫缺陷样病毒（BIV）的犊牛中研究了BIV的早期致病作用。所有接种BIV R29感染细胞的动物在接种后6周血清转化，并且在接种后2周可从每只动物中分离出BIV。然而，BIV在体内的复制水平似乎较低。原位杂交研究表明，在体内病毒复制的高峰期，不到0.03%的外周血单核细胞表达可检测水平的病毒RNA。此外，这些细胞在体内的病毒RNA水平不到体外持续感染细胞中观察到水平的1/10。接种BIV的犊牛循环淋巴细胞数量显著增加，并且在淋巴结、血淋巴结和脾脏中观察到滤泡增生。在感染BIV的犊牛中观察到的组织病理学变化与感染免疫抑制性慢病毒（包括1型人类免疫缺陷病毒）后早期发现的变化相似。
