Marich J E, Pontsler A V, Rice S M, McGraw K A, Dubensky T W
Syngene Inc., San Diego, California 92121.
Virology. 1992 Feb;186(2):770-6. doi: 10.1016/0042-6822(92)90045-q.
The 7851-bp nucleotide sequence of human papillomavirus (HPV) type 35 was determined. HPV 35 is associated with high-grade cervical intraepithelial neoplasia and invasive carcinomas. From the HPV 35 sequence, open reading frames encoding putative proteins E6, E7, E1, E2, E4, E5, L2, and L1, common to other mucosal HPV types, were identified. Structural and control elements present in the long control region (LCR) conserved among other mucosal HPV types were also present in HPV 35. Analysis of the LCR revealed an additional 20-bp sequence element present in all HPV types associated with malignant proliferation. To further classify HPV 35 with regard to oncogenic potential, phylogenetic analysis of the E6 and E7 proteins from the anogenital HPV types 6, 11, 16, 18, 31, 33, 35, 39, 43, 44, 45, and 51 was performed. This analysis indicated three distinct HPV subgroups; those associated with benign lesions and two branches of those HPV types more often associated with malignant proliferation. HPV 35 is most closely related to HPV types 31 and 16.
测定了人乳头瘤病毒（HPV）35型的7851个碱基对的核苷酸序列。HPV 35与高级别宫颈上皮内瘤变和浸润性癌相关。从HPV 35序列中，鉴定出了与其他黏膜型HPV共有的、编码推定蛋白E6、E7、E1、E2、E4、E5、L2和L1的开放阅读框。其他黏膜型HPV中长控制区（LCR）存在的结构和调控元件在HPV 35中也存在。对LCR的分析揭示了所有与恶性增殖相关的HPV类型中都存在的一个额外的20个碱基对的序列元件。为了进一步根据致癌潜力对HPV 35进行分类，对肛门生殖器HPV类型6、11、16、18、31、33、35、39、43、44、45和51的E6和E7蛋白进行了系统发育分析。该分析表明存在三个不同的HPV亚组；那些与良性病变相关的亚组以及那些更常与恶性增殖相关的HPV类型的两个分支。HPV 35与HPV 31型和16型关系最为密切。
