Eriksson A, Herron J R, Yamada T, Wheeler C M
J Gen Virol. 1999 Mar;80 ( Pt 3):595-600. doi: 10.1099/0022-1317-80-3-595.
We have previously examined 29 cervical cell isolates for human papillomavirus type 16 (HPV-16) sequence variations in the E6, L2 and L1 coding regions, and the long control region (LCR). Twenty-five of these isolates as well as 23 additional isolates are characterized here as we present the complete E5 coding segment and the E2 hinge region. Eight amino acid variations were observed in the E5 coding segment, 13 were identified in the E2 hinge region and 5 were observed in the overlapping E4 coding segment. These amino acid variations may be relevant to differences in biological functions and may result in altered humoral or cell-mediated immune responses to HPV-16 variants. The characterization of sequence variation within high-risk HPV types might be important in the search for epidemiological correlates of cervical cancer risk. This work complements and extends HPV-16 genome sequence information from specific isolates previously reported by our group.
我们之前已对29株宫颈细胞分离株的人乳头瘤病毒16型（HPV - 16）的E6、L2和L1编码区以及长控制区（LCR）的序列变异情况进行了检测。在此，我们展示完整的E5编码片段和E2铰链区，对其中25株分离株以及另外23株分离株进行了特征分析。在E5编码片段中观察到8个氨基酸变异，在E2铰链区鉴定出13个变异，在重叠的E4编码片段中观察到5个变异。这些氨基酸变异可能与生物学功能差异相关，并可能导致对HPV - 16变异体的体液或细胞介导免疫反应发生改变。高危型HPV类型内序列变异的特征分析对于寻找宫颈癌风险的流行病学关联可能具有重要意义。这项工作补充并扩展了我们小组之前报道的特定分离株的HPV - 16基因组序列信息。
