Tan C H, Tachezy R, Van Ranst M, Chan S Y, Bernard H U, Burk R D
Laboratory for Papillomavirus Biology, National University of Singapore.
Virology. 1994 Feb;198(2):534-41. doi: 10.1006/viro.1994.1064.
Mastomys natalensis is a rodent of African origin afflicted with a very high incidence of skin tumors (keratoacanthomas and squamous carcinomas), which are associated with a papillomavirus, M. natalensis papillomavirus (MnPV). We have determined the genomic sequence of MnPV, which has a size of 7687 bp. The genomic organization is similar to that of other papillomaviruses, with open reading frames E6, E7, E1, E2, and E4 in the early and L2 and L1 in the late region. Due to an unusually large hinge region, the transcriptional activator E2 has a size of 542 amino acids rather than 400 to 460 amino acids, as in other papillomaviruses. An open reading frame E5 coding for a small hydrophobic membrane protein is missing, as is the case for some cutaneous human papillomaviruses (HPV). This fact, together with the composition of cis-responsive elements in its long control region and phylogenetic evaluation of segments of its E6, E1, and L1 genes, indicates a relationship of MnPV to the cottontail rabbit papillomavirus and several HPV types found in lesions of cutaneous epithelia, in particular to those that are associated with epidermodysplasia verruciformis. MnPV may be a useful model system for tumorigenesis of cutaneous epithelia in humans.
南非多乳鼠是一种原产于非洲的啮齿动物，其皮肤肿瘤（角化棘皮瘤和鳞状细胞癌）的发病率非常高，这些肿瘤与一种乳头瘤病毒——南非多乳鼠乳头瘤病毒（MnPV）有关。我们已经确定了MnPV的基因组序列，其大小为7687 bp。基因组结构与其他乳头瘤病毒相似，早期区域有开放阅读框E6、E7、E1、E2和E4，晚期区域有L2和L1。由于铰链区异常大，转录激活因子E2有542个氨基酸，而不像其他乳头瘤病毒那样有400至460个氨基酸。一个编码小疏水膜蛋白的开放阅读框E5缺失，一些皮肤型人乳头瘤病毒（HPV）也是如此。这一事实，连同其长控制区顺式反应元件的组成以及其E6、E1和L1基因片段的系统发育评估，表明MnPV与棉尾兔乳头瘤病毒以及在皮肤上皮病变中发现的几种HPV类型有关，特别是与那些与疣状表皮发育不良相关的类型有关。MnPV可能是人类皮肤上皮肿瘤发生的一个有用模型系统。
