Wild G E, Murray D
Department of Medicine, McGill University, Montreal, Canada.
Dig Dis Sci. 1992 Mar;37(3):417-25. doi: 10.1007/BF01307737.
The distribution of sodium- and potassium-stimulated ATPase (Na,K-ATPase) along the crypt-villus axis and crypt cytokinetics were examined in an infective model of celiac disease produced by infection of the rat with the nematode Nippostrongylus brasiliensis. In controls, levels of enzyme activity remained stable during enterocyte migration to the villous apex. In the jejunum of infected rats, the structural lesion of villous atrophy and crypt hyperplasia, observed at day 10 of infection, was associated with a three-dimensional expansion of the crypts. Cell cycle time was shortened and this resulted in a markedly increased crypt cell production rate. Enterocytes emerged from the crypts at a faster rate, and this functional immaturity was paralleled by decreased Na,K-ATPase activity. Further decreases in enzyme levels were observed during enterocyte migration along the villi. This may reflect enterocyte damage or increased enzyme turnover. In the ileum of these animals, enterocyte maturation was prolonged and enzyme activity was increased at the level of the crypt villus junction with further increases noted during enterocyte transit. These changes in ileal Na,K-ATPase appear to be adaptive.
在通过用巴西日圆线虫感染大鼠所建立的腹腔疾病感染模型中，研究了钠钾刺激的ATP酶（Na，K - ATP酶）沿隐窝 - 绒毛轴的分布以及隐窝细胞动力学。在对照组中，肠上皮细胞迁移至绒毛顶端的过程中，酶活性水平保持稳定。在感染大鼠的空肠中，感染第10天观察到的绒毛萎缩和隐窝增生的结构损伤与隐窝的三维扩张有关。细胞周期时间缩短，这导致隐窝细胞产生率显著增加。肠上皮细胞从隐窝中出现的速度更快，这种功能不成熟伴随着Na，K - ATP酶活性降低。在肠上皮细胞沿绒毛迁移过程中，观察到酶水平进一步下降。这可能反映了肠上皮细胞损伤或酶周转增加。在这些动物的回肠中，肠上皮细胞成熟延长，在隐窝 - 绒毛交界处酶活性增加，在肠上皮细胞转运过程中进一步增加。回肠中Na，K - ATP酶的这些变化似乎是适应性的。
