Mendiola M V, Jubete Y, de la Cruz F
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
J Bacteriol. 1992 Feb;174(4):1345-51. doi: 10.1128/jb.174.4.1345-1351.1992.
IS91 is a 1,830-bp insertion sequence that inserts specifically at the sequence CAAG or GAAC of the target and does not duplicate any sequence upon insertion (23). By transposon mutagenesis, we have identified open reading frame 426 (ORF426; bp 454 to 1731) as the putative ORF for the transposase. It displays a cysteine-rich, potential metal-binding domain in its N-terminal region. Adjacent to ORF426, there is an ORF (ORF121) which precedes and terminally overlaps ORF426 by one amino acid. Tn1732 insertions in ORF121 do not affect the transposition frequency. IS91 has sequence similarities to IS801 from Pseudomonas syringae. Their putative transposases are 36% identical, including conservation of the cysteine-rich cluster. The information concerning IS801 insertion specificity and target duplication has been reevaluated in the light of our results.
IS91是一个1830碱基对的插入序列，它特异性地插入到靶序列的CAAG或GAAC处，插入时不复制任何序列（23）。通过转座子诱变，我们已将开放阅读框426（ORF426；碱基对454至1731）鉴定为转座酶的推定开放阅读框。它在其N端区域显示出一个富含半胱氨酸的潜在金属结合结构域。与ORF426相邻的是一个开放阅读框（ORF121），它在ORF426之前，并且在末端与ORF426重叠一个氨基酸。ORF121中的Tn1732插入不影响转座频率。IS91与丁香假单胞菌的IS801具有序列相似性。它们推定的转座酶有36%的同一性，包括富含半胱氨酸簇的保守性。根据我们的结果，已重新评估了有关IS801插入特异性和靶序列重复的信息。
