pUR 250可对其插入片段的两条DNA链进行快速化学测序。
pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.
作者信息
Rüther U
出版信息
Nucleic Acids Res. 1982 Oct 11;10(19):5765-72. doi: 10.1093/nar/10.19.5765.
Abstract
A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment.
摘要
构建了一种多用途质粒pUR 250。它在其lac Z基因部分的一个小区域内含有七个独特的克隆位点（Hind III、Xba I、Sal I、Acc I、Hinc II、BamH I和EcoR I）。将外源DNA插入质粒很容易被检测到。通过快速方法从克隆中分离出的质粒DNA可直接用于从两端确定插入片段的DNA序列，而无需分离标记片段。
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