Zabala J C, García-Lobo J M, Diaz-Aroca E, de la Cruz F, Ortiz J M
Mol Gen Genet. 1984;197(1):90-7. doi: 10.1007/BF00327927.
A revised physical map of the alpha-haemolysin plasmid pHly152 has been constructed. The known position of the hly genes in the restriction map of pHly152 allowed us to locate in it a direct repeat of IS elements flanking the hly genes of pHly152. These elements are IS92L, which is a derivative of the previously characterised element IS91 (1.85 kb) by insertion of a sequence of 1.2 kb, and IS92R, an element related to IS91 by a deletion of 0.7 kb and substitution of a 0.2 kb sequence of IS91 by a 1.2 kb heterologous sequence. IS92L is, in turn, flanked by an inverted repetition of sequences of 1.4 kb. These and previously published data strongly suggest that the hly genes spread at some time in evolution by means of the recombinational activity of IS91-like elements.
已构建了α-溶血素质粒pHly152的修订物理图谱。pHly152限制性图谱中hly基因的已知位置使我们能够在其中定位到pHly152的hly基因两侧IS元件的直接重复序列。这些元件是IS92L，它是通过插入1.2 kb的序列从先前鉴定的元件IS91（1.85 kb）衍生而来；以及IS92R，它与IS91相关，是通过缺失0.7 kb并将IS91的0.2 kb序列替换为1.2 kb的异源序列而形成的。IS92L又被1.4 kb序列的反向重复所包围。这些以及先前发表的数据有力地表明，hly基因在进化的某个时期通过类IS91元件的重组活性得以传播。
