Bölker M, Urban M, Kahmann R
Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Cell. 1992 Feb 7;68(3):441-50. doi: 10.1016/0092-8674(92)90182-c.
The a mating type locus of the phytopathogenic fungus U. maydis controls fusion of haploid cells and filamentous growth of the dikaryotic mycelium. The a locus exists in two alleles, termed a1 and a2, which are defined by nonhomologous DNA regions comprising 4.5 kb for a1 and 8 kb for a2, flanked by identical sequences. Based on functional assays, mutants, and sequencing, we demonstrate that the mating type in each allele is determined by a set of two genes. One encodes a precursor for a lipopeptide mating factor, and the other specifies the receptor for the pheromone secreted by cells of opposite mating type. Thus, U. maydis employs a novel strategy to determine its mating type by providing the primary determinants of cell-cell recognition directly from the mating type locus.
植物致病真菌玉米黑粉菌的a交配型位点控制单倍体细胞的融合以及双核菌丝体的丝状生长。a位点存在两个等位基因，称为a1和a2，它们由非同源DNA区域定义，a1包含4.5 kb，a2包含8 kb，两侧为相同序列。基于功能分析、突变体和测序，我们证明每个等位基因中的交配型由一组两个基因决定。一个基因编码脂肽交配因子的前体，另一个基因指定相反交配型细胞分泌的信息素的受体。因此，玉米黑粉菌采用了一种新策略，即直接从交配型位点提供细胞间识别的主要决定因素来确定其交配型。
