Guengerich F P, Broquist H P
J Bacteriol. 1976 Apr;126(1):338-47. doi: 10.1128/jb.126.1.338-347.1976.
The catabolism of lysine was studied in several yeasts and fungi. Results with cell-free extracts of Rhizoctonia leguminicola support a proposed pathway involving (D- and L-) EPSILON-N-acetyllysine, alpha-keto-epsilon-acetamidohexanoic acid, delta-acetamidovaleric acid, and delta-aminovaleric acid in the conversion of L-lysine to shortchain organic acids. Label from radioactive L-lysine was found to accumulate in D- and L-epsilon-N-acetyllysine, delta-acetamidovaleric acid, delta-aminovaleric acid, and glutaric acid in cultures of R. leguminicola, Neurospora crassa, Saccharomyces cerevisiae, and Hansenula saturnus, suggesting that the proposed omega-acetyl pathway of lysine catabolism is generalized among yeasts and fungi. In N. crassa, as is the case in R. leguminicola, the major precursor of L-pipecolic acid was the L-isomer of lysine; 15N experiments were consistent with delta1-piperideine-2-carboxylic acid as an intermediate in the transformation.
在几种酵母和真菌中研究了赖氨酸的分解代谢。豆科丝核菌无细胞提取物的结果支持了一条推测的途径，该途径涉及在将L-赖氨酸转化为短链有机酸的过程中，（D-和L-）ε-N-乙酰赖氨酸、α-酮-ε-乙酰氨基己酸、δ-乙酰氨基戊酸和δ-氨基戊酸。在豆科丝核菌、粗糙脉孢菌、酿酒酵母和土星汉逊酵母的培养物中，发现放射性L-赖氨酸的标记物在D-和L-ε-N-乙酰赖氨酸、δ-乙酰氨基戊酸、δ-氨基戊酸和戊二酸中积累，这表明推测的赖氨酸分解代谢的ω-乙酰途径在酵母和真菌中普遍存在。在粗糙脉孢菌中，与豆科丝核菌的情况一样，L-哌啶酸的主要前体是赖氨酸的L-异构体；15N实验与δ1-哌啶-2-羧酸作为转化过程中的中间体一致。
