Chang K H, Day C, Walker J, Hyypiä T, Stanway G
Department of Biology, University of Essex, U.K.
J Gen Virol. 1992 Mar;73 ( Pt 3):621-6. doi: 10.1099/0022-1317-73-3-621.
We have shown previously that, compared to other enteroviruses, the coxsackievirus A9 (CAV-9) prototype strain, Griggs, contains a C-terminal extension to the capsid protein VP1 and that within this extension there is an RGD (arginine-glycine-aspartic acid) motif. To determine whether these features are found in other CAV-9 strains and therefore analyse whether they are likely to be functionally important, we have determined the nucleotide sequence of the appropriate region from five strains, isolated over a 25 year period. The results indicate that there is considerable diversity between the strains and there is little correlation between nucleotide sequence identity and date of isolation. All isolates exhibit the VP1 extension and although its amino acid sequence is otherwise variable, the RGD motif is common to all. This conservation of sequence, within a region which can otherwise vary, implies that the RGD sequence must be functionally significant. The VP1 extension shows similarity to sequences found in foot-and-mouth-disease virus strains and to part of the precursor of the cellular protein, human transforming growth factor beta, and the possible significance of these observations is discussed.
我们之前已经表明，与其他肠道病毒相比，柯萨奇病毒A9（CAV-9）原型株格里格斯（Griggs）的衣壳蛋白VP1含有一个C末端延伸，并且在这个延伸区域内有一个RGD（精氨酸-甘氨酸-天冬氨酸）基序。为了确定这些特征是否存在于其他CAV-9毒株中，并因此分析它们是否可能具有功能重要性，我们测定了在25年期间分离的5个毒株相应区域的核苷酸序列。结果表明，这些毒株之间存在相当大的差异，核苷酸序列同一性与分离日期之间几乎没有相关性。所有分离株都表现出VP1延伸，尽管其氨基酸序列在其他方面存在变异，但RGD基序在所有分离株中都很常见。在一个其他方面可能会变化的区域内这种序列的保守性意味着RGD序列必定具有功能重要性。VP1延伸与口蹄疫病毒株中发现的序列以及细胞蛋白人转化生长因子β前体的一部分具有相似性，并对这些观察结果的可能意义进行了讨论。
