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来自枯草芽孢杆菌壮观霉素抗性突变体的蛋白质S5中的氨基酸替换

Amino acid replacement in the protein S5 from a spectinomycin resistant mutant of Bacillus subtilis.

作者信息

Itoh T

出版信息

Mol Gen Genet. 1976 Feb 27;144(1):39-42. doi: 10.1007/BF00277301.

DOI:10.1007/BF00277301
PMID:131241
Abstract

Ribosomal protein S5 was isolated from wild type Bacillus subtilis ATCC 6633 and from a spectinomycin resistant mutant (BSPC 111) derived from spectinomycin sensitive to resistance is accomtrypsin and all the tryptic peptides were isolated by column- and paper-chromatography. By comparative amino acid analyses of the peptides, it was demonstrated that the S5 from the mutant differs from the wild type S5 by a replacement of one amino acid, namely lysine by isoleucine in the peptide T9. The results are compared with E. coli spectinomycin resistant mutants.

摘要

核糖体蛋白S5是从野生型枯草芽孢杆菌ATCC 6633以及从对壮观霉素敏感到抗性的突变体（BSPC 111）中分离得到的，该突变体对壮观霉素产生抗性，经胰蛋白酶处理后，所有胰蛋白酶肽段通过柱色谱和纸色谱进行分离。通过对这些肽段的氨基酸比较分析表明，突变体中的S5与野生型S5的差异在于一个氨基酸的替换，即在肽段T9中赖氨酸被异亮氨酸取代。将这些结果与大肠杆菌壮观霉素抗性突变体进行了比较。

相似文献

1
Amino acid replacement in the protein S5 from a spectinomycin resistant mutant of Bacillus subtilis.来自枯草芽孢杆菌壮观霉素抗性突变体的蛋白质S5中的氨基酸替换
Mol Gen Genet. 1976 Feb 27;144(1):39-42. doi: 10.1007/BF00277301.
2
Alteration of ribosomal protein S5 from two spectinomycin-resistant mutants of Bacillus subtilis: deletion of one amino acid residue.枯草芽孢杆菌两个抗壮观霉素突变体中核糖体蛋白S5的改变：一个氨基酸残基的缺失。
Mol Gen Genet. 1982;185(2):205-6. doi: 10.1007/BF00330787.
3
Mutation affecting expression of spectinomycin resistance in Bacillus subtilis.影响枯草芽孢杆菌中壮观霉素抗性表达的突变。
J Bacteriol. 1980 Jan;141(1):409-12. doi: 10.1128/jb.141.1.409-412.1980.
4
Ribosomal proteins from streptomycin-resistant and dependent mutants, and revertants from streptomycin-dependence to independence in Bacillus subtilis.来自枯草芽孢杆菌中链霉素抗性和依赖性突变体以及从链霉素依赖性回复到非依赖性的回复子的核糖体蛋白。
Mol Gen Genet. 1975 Sep 8;139(4):293-301. doi: 10.1007/BF00267969.
5
Ribosomal proteins. XXII. Studies on the altered protein S5 from a spectinomycin-resistant mutant of Escherichia coli.核糖体蛋白。二十二。对来自大肠杆菌壮观霉素抗性突变体的改变的蛋白S5的研究。
J Mol Biol. 1972 Feb 28;64(1):201-9. doi: 10.1016/0022-2836(72)90329-4.
6
Genetic position and amino acid replacements of several mutations in ribosomal protein S5 from Escherichia coli.来自大肠杆菌的核糖体蛋白S5中几个突变的基因位置和氨基酸替换
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Mol Gen Genet. 1973 Aug 10;124(2):107-15. doi: 10.1007/BF00265144.
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Localization of the amino-acid exchange in protein S5 from an Escherichia coli mutant resistant to spectinomycin.来自一株对壮观霉素耐药的大肠杆菌突变体中蛋白质S5氨基酸交换的定位。
Mol Gen Genet. 1973 Dec 31;127(3):273-6. doi: 10.1007/BF00333767.
10
Spectinomycin dependence in Bacillus subtilis.枯草芽孢杆菌对壮观霉素的依赖性。
J Bacteriol. 1979 Mar;137(3):1452-5. doi: 10.1128/jb.137.3.1452-1455.1979.

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本文引用的文献

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Ribosomal Protein Conferring Sensitivity to the Antibiotic Spectinomycin in Escherichia coli.赋予大肠杆菌对抗生素壮观霉素敏感性的核糖体蛋白
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Mutation affecting expression of spectinomycin resistance in Bacillus subtilis.影响枯草芽孢杆菌中壮观霉素抗性表达的突变。
J Bacteriol. 1980 Jan;141(1):409-12. doi: 10.1128/jb.141.1.409-412.1980.
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Alteration of ribosomal protein S5 from two spectinomycin-resistant mutants of Bacillus subtilis: deletion of one amino acid residue.枯草芽孢杆菌两个抗壮观霉素突变体中核糖体蛋白S5的改变：一个氨基酸残基的缺失。
Mol Gen Genet. 1982;185(2):205-6. doi: 10.1007/BF00330787.
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Revised genetic linkage map of Bacillus subtilis.枯草芽孢杆菌的修订遗传连锁图谱。
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Eur J Biochem. 1971 Nov 11;23(1):7-11. doi: 10.1111/j.1432-1033.1971.tb01584.x.
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Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.核糖体蛋白。VII. 用于核糖体蛋白指纹图谱分析的二维聚丙烯酰胺凝胶电泳
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Genetic studies of the ribosomal proteins in Escherichia. coli. II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants.大肠杆菌核糖体蛋白的遗传学研究。II. 对壮观霉素抗性突变体特异的30S核糖体蛋白组分的改变
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Amino acid replacements in proteins S5 and S12 of two Escherichia coli revertants from streptomycin dependence to independence.两个从链霉素依赖型回复为非依赖型的大肠杆菌回复突变体中蛋白质S5和S12的氨基酸替换。
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Ribosomal proteins. XXVII. Localization of the amino acid exchanges in protein S5 from two Escherichia coli mutants resistant to spectinomycin.核糖体蛋白。XXVII. 来自两种抗壮观霉素的大肠杆菌突变体的蛋白S5中氨基酸交换的定位。
Mol Gen Genet. 1972;114(2):106-11. doi: 10.1007/BF00332781.
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Localization of the amino-acid exchange in protein S5 from an Escherichia coli mutant resistant to spectinomycin.来自一株对壮观霉素耐药的大肠杆菌突变体中蛋白质S5氨基酸交换的定位。
Mol Gen Genet. 1973 Dec 31;127(3):273-6. doi: 10.1007/BF00333767.
9
Genetic and chemical studies of ribosomes from spectinomycin resistant mutants of Bacillus subtilis.枯草芽孢杆菌壮观霉素抗性突变体核糖体的遗传与化学研究。
Mol Gen Genet. 1973 Aug 10;124(2):107-15. doi: 10.1007/BF00265144.
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Ribosomal proteins. XXII. Studies on the altered protein S5 from a spectinomycin-resistant mutant of Escherichia coli.核糖体蛋白。二十二。对来自大肠杆菌壮观霉素抗性突变体的改变的蛋白S5的研究。
J Mol Biol. 1972 Feb 28;64(1):201-9. doi: 10.1016/0022-2836(72)90329-4.