Dombou M, Mizuno T, Mizushima S
Mol Gen Genet. 1977 Sep 21;155(1):53-60. doi: 10.1007/BF00268560.
Alterations in the ribosomes of sucrose-dependent spectinomycin-resistant (Sucd-Spcr) mutants of Escherichia coli were studied. Subunit exchange experiments showed that 30S subunits were responsible for the resistance of ribosomes to spectinomycin in all Sucd-Spcr mutants tested. Proteins of 30S ribosomes were analyzed by carboxymethyl cellulose column chromatography based on their elution positions. Mutants YM22 and YM93 had an altered 30S ribosomal protein component, S5, and mutant YM50 had an altered protein, S4. Although a shift of elution position was not detected for all the 30S ribosomal proteins from mutant YM101, the amount of protein S3 was appreciably lowered in the isolated 30S subunits. A partial reconstitution experiment with protein S3 prepared from both the wild-type strain and YM101 revealed that the mutant had altered protein S3 which is responsible for the spectinomycin resistance. These alterations in 30S subunits are discussed in relation to the interaction between ribosomes and the cytoplasmic membrane.
对大肠杆菌蔗糖依赖性壮观霉素抗性（Sucd-Spcr）突变体核糖体的变化进行了研究。亚基交换实验表明，在所有测试的Sucd-Spcr突变体中，30S亚基负责核糖体对壮观霉素的抗性。基于洗脱位置，通过羧甲基纤维素柱色谱法分析了30S核糖体的蛋白质。突变体YM22和YM93的30S核糖体蛋白组分S5发生了改变，突变体YM50的蛋白S4发生了改变。尽管未检测到突变体YM101所有30S核糖体蛋白的洗脱位置发生变化，但在分离的30S亚基中，蛋白S3的量明显降低。用野生型菌株和YM101制备的蛋白S3进行的部分重组实验表明，该突变体的蛋白S3发生了改变，这与壮观霉素抗性有关。讨论了30S亚基的这些变化与核糖体和细胞质膜之间相互作用的关系。
