Kamath Divya, Gregory Steven T, O'Connor Michael
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, Missouri, USA.
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA.
Antimicrob Agents Chemother. 2017 Jan 24;61(2). doi: 10.1128/AAC.01186-16. Print 2017 Feb.
Ribosomal protein uS5 is an essential component of the small ribosomal subunit that is involved in subunit assembly, maintenance of translational fidelity, and the ribosome's response to the antibiotic spectinomycin. While many of the characterized uS5 mutations that affect decoding map to its interface with uS4, more recent work has shown that residues distant from the uS4-uS5 interface can also affect the decoding process. We targeted one such interface-remote area, the loop 2 region (residues 20 to 31), for mutagenesis in Escherichia. coli and generated 21 unique mutants. A majority of the loop 2 alterations confer resistance to spectinomycin and affect the fidelity of translation. However, only a minority show altered rRNA processing or ribosome biogenesis defects.
核糖体蛋白uS5是小核糖体亚基的重要组成部分，参与亚基组装、维持翻译保真度以及核糖体对抗生素壮观霉素的反应。虽然许多已鉴定的影响解码的uS5突变位于其与uS4的界面处，但最近的研究表明，远离uS4 - uS5界面的残基也会影响解码过程。我们针对一个这样的远离界面的区域，即环2区域（第20至31位残基），在大肠杆菌中进行诱变，并产生了21个独特的突变体。环2的大多数改变赋予了对壮观霉素的抗性，并影响翻译保真度。然而，只有少数显示出rRNA加工改变或核糖体生物发生缺陷。
