Blakeslee D, Baines M G
J Immunol Methods. 1976;13(3-4):305-20. doi: 10.1016/0022-1759(76)90078-8.
Dichlorotriazinylaminofluorescein (DTAF), the product of the reaction of aminofluorescein with cyanuric chloride, is an effective reagent for conjugating fluorescein to immunnoglobulins. DTAF has absorption and emission properties nearly identical to fluoresceinisothiocyanate (FITC) and DTAF and FITC-labelled antibodies are similar in terms of preparation and specificity of immlnofluorescence. However, DTAF is superior to FITC with regard to cost, purity and stability. Also, DTAF-labelled rabbit IgG conjugates can by quickly and efficientyly fractionated by simple ammonium sulfate precipitation procedures to yield preparations free of both over- and under-conjugated material. In addition, over-conjugated protein can be readily removed from DTAF:IgG conjugates by appropriate adjustment of pH and temperature.
二氯三嗪基氨基荧光素（DTAF）是氨基荧光素与三聚氯氰反应的产物，是一种将荧光素与免疫球蛋白结合的有效试剂。DTAF具有与异硫氰酸荧光素（FITC）几乎相同的吸收和发射特性，并且DTAF和FITC标记的抗体在免疫荧光的制备和特异性方面相似。然而，在成本、纯度和稳定性方面，DTAF优于FITC。此外，DTAF标记的兔IgG结合物可以通过简单的硫酸铵沉淀程序快速有效地分级分离，以得到不含过度结合和结合不足物质的制剂。另外，通过适当调节pH值和温度，可以很容易地从DTAF:IgG结合物中去除过度结合的蛋白质。
