Schilt U
Z Immunitatsforsch Immunobiol. 1979 Jun;155(5):411-9.
While evaluating herpes simplex virus (HSV) typing by indirect immunofluorescence staining, an undesired specific staining pattern turned out to be a reliable marker for herpes simplex type 1. Cells infected with herpes simplex type 1 displayed clear staining with a FITC-conjugated antiglobulin preparation, also in the absence of herpes simplex-specific antibodies. Using the same conjugate, herpes simplex type-2-infected cells exhibited no fluorescence. The particular type of staining observed was influenced by neither the anatomical site of origin of the virus isolate nor the cell type used for virus preparation. Herpes simplex type 1-specific fluorescence was only obtained with the use of FITC-conjugates possessing anti-IgG activity. Both reliability and specificity of this discriminating procedure as a diagnostic tool has been established by typing 282 virus isolates over a period of 4 years.
在通过间接免疫荧光染色评估单纯疱疹病毒（HSV）分型时，一种不期望出现的特异性染色模式被证明是1型单纯疱疹病毒的可靠标志物。感染1型单纯疱疹病毒的细胞，即使在没有单纯疱疹特异性抗体的情况下，用异硫氰酸荧光素（FITC）偶联的抗球蛋白制剂染色时也会显示清晰的染色。使用相同的偶联物时，感染2型单纯疱疹病毒的细胞无荧光显示。观察到的这种特殊染色类型不受病毒分离株的解剖学来源部位或用于病毒制备的细胞类型的影响。只有使用具有抗IgG活性的FITC偶联物才能获得1型单纯疱疹病毒特异性荧光。在4年的时间里对282株病毒分离株进行分型，确立了这种鉴别方法作为诊断工具的可靠性和特异性。
