人肝细胞生长因子刺激人肝癌细胞系HUH-6克隆5细胞的生长。
Human hepatocyte growth factor stimulates the growth of HUH-6 clone 5 human hepatoblastoma cells.
作者信息
Miyazaki M, Gohda E, Tsuboi S, Tsubouchi H, Daikuhara Y, Namba M, Yamamoto I
机构信息
Department of Cell Biology, Okayama University Medical School, Japan.
出版信息
Cell Biol Int Rep. 1992 Feb;16(2):145-54. doi: 10.1016/s0309-1651(06)80108-6.
The effects of human hepatocyte growth factor (hHGF), a potent mitogen for rat and human hepatocytes in primary culture, on proliferation of human hepatoma and hepatoblastoma cells were examined. Out of five cell lines; HLE, HuH-6 clone 5, HuH-7, PLC/PRF/5, and Hep G2, only HuH-6 Clone 5 cells were stimulated by recombinant hHGF. Both native and recombinant hHGFs caused dose-dependent increases in cell number and DNA synthesis of cells. This stimulation was strongly inhibited by anti-hHGF monoclonal antibody.
研究了人肝细胞生长因子（hHGF）对人肝癌细胞和肝母细胞瘤细胞增殖的影响，hHGF是原代培养大鼠和人肝细胞的一种有效促有丝分裂原。在5种细胞系（HLE、HuH-6克隆5、HuH-7、PLC/PRF/5和Hep G2）中，只有HuH-6克隆5细胞受到重组hHGF的刺激。天然和重组hHGF均导致细胞数量和细胞DNA合成呈剂量依赖性增加。这种刺激被抗hHGF单克隆抗体强烈抑制。
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