Kobayashi T K
Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation, Inc., Japan.
Diagn Cytopathol. 1992;8(1):53-60. doi: 10.1002/dc.2840080110.
Over a 62-month period, 53 patients were found to have cervicovaginal smears that contained cells consistent with, or equivocal cells for, a herpes simplex virus (HSV) infection. The Papanicolaou-destained smears from these cases were restrained in situ hybridization (ISH) with a biotinylated cloned DNA probe and immunocytochemistry (ICC) assay and were compared for the detection of HSV in cervicovaginal smears by two methods. Cytological findings classified the 53 slides into two groups, i.e., cytologically herpes positive (33 patients) and equivocal cases (20 patients). Each group was subdivided into two groups: group A was confirmed by ICC, and group B was confirmed by ISH technique. Of the 33 cellular samples containing cells considered to be consistent with a herpes infection, 15 (88%) of 17 were positive by means of ICC technique (group A), 6 (43%) of 14 were positive by ISH technique (group B). Of the 20 smears showing equivocal cell changes thought unlikely to be caused by an HSV infection, 6 (60%) of 10 were positive by ICC (group A), 2 (29%) of 7 were positive by ISH (group B). With the ISH technique, five smears showed dislodged cells from glass slides due to enzyme treatment and denaturation. The results revealed that the ICC technique is a rapid and reliable procedure and thus recommended for routine diagnosis of HSV infection. Moreover, ICC is easier to perform and interpret and is less expensive than ISH. Therefore, the ICC may be preferable to ISH for detecting HSV in routine Papanicolaou diagnostic work.
在62个月的时间里，发现53例患者的宫颈阴道涂片含有与单纯疱疹病毒（HSV）感染相符的细胞或可疑细胞。对这些病例经巴氏染色的涂片进行生物素化克隆DNA探针原位杂交（ISH）和免疫细胞化学（ICC）检测，并比较两种方法对宫颈阴道涂片中HSV的检测情况。细胞学检查结果将53张涂片分为两组，即细胞学上疱疹阳性（33例患者）和可疑病例（20例患者）。每组又分为两组：A组通过ICC确诊，B组通过ISH技术确诊。在33份含有被认为与疱疹感染相符细胞的细胞样本中，17份中的15份（88%）通过ICC技术呈阳性（A组），14份中的6份（43%）通过ISH技术呈阳性（B组）。在20份显示可疑细胞变化且认为不太可能由HSV感染引起的涂片中，10份中的6份（60%）通过ICC呈阳性（A组），7份中的2份（29%）通过ISH呈阳性（B组）。采用ISH技术时，有5份涂片因酶处理和变性导致细胞从载玻片上脱落。结果显示，ICC技术是一种快速可靠的方法，因此推荐用于HSV感染的常规诊断。此外，ICC操作和解读更简便，且比ISH成本更低。因此，在常规巴氏诊断工作中检测HSV时，ICC可能比ISH更可取。
